Search results

• 2024

  A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations

  Anderson, M. A. E., Gonzalez, E., Edgington, M. P., Ang, J. X. D., Purusothaman, D. K., Shackleford, L., Nevard, K., Verkuijl, S. A. N., Harvey-Samuel, T., Leftwich, P. T., Esvelt, K. & Alphey, L., 25 Jan 2024, In: Nature Communications. 15, 729.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  5 Citations (Scopus)

  6 Downloads (Pure)

• A synthetic biology approach to transgene expression in insects

  Leftwich, P. T., Purcell, J. C., Anderson, M. A. E., Fragkoudis, R., Basu, S., Lycett, G. & Alphey, L., 28 Aug 2024, (E-pub ahead of print) In: ACS Synthetic Biology.

  Research output: Contribution to journal › Article › peer-review

  Open Access

• Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata

  Meccariello, A., Hou, S., Davydova, S., Fawcett, J. D., Siddall, A., Leftwich, P. T., Krsticevic, F., Papathanos, P. A. & Windbichler, N., 8 Jan 2024, In: Nature Communications. 15, 372.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  2 Citations (Scopus)

  14 Downloads (Pure)
• 2023

  Accentuating the positive and eliminating the negative: Efficacy of TiO₂ as digestibility index marker for poultry nutrition studies

  Sprigg, C., Leftwich, P. T., Burton, E., Scholey, D., Bedford, M. R. & Brearley, C. A., 26 Jun 2023, In: PLoS One. 18, 6, e0284724.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  6 Downloads (Pure)

• AePUb promoter length modulates gene expression in Aedes aegypti

  Anderson, M. A. E., Leftwich, P. T., Wilson, R., Carabajal Paladino, L. Z., Basu, S., Rooney, S., Adelman, Z. N. & Alphey, L., 21 Nov 2023, In: Scientific Reports. 13, 20352.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  1 Citation (Scopus)

  4 Downloads (Pure)

• A synthetic biology approach to transgene expression

  Leftwich, P. T., Purcell, J. C., Anderson, M. A. E., Fragkoudis, R., Basu, S., Lycett, G. T. & Alphey, L., 31 Aug 2023.

  Research output: Working paper › Preprint

• CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus

  Harvey-Samuel, T., Feng, X., Okamoto, E. M., Purusothaman, D-K., Leftwich, P. T., Alphey, L. & Gantz, V. M., 20 Nov 2023, In: Nature Communications. 14, 7561.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  3 Downloads (Pure)

• Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti

  Carabajal Paladino, L. Z., Wilson, R., Tng, P. Y. L., Dhokiya, V., Keen, E., Cuber, P., Larner, W., Rooney, S., Nicholls, M., Uglow, A., Williams, L., Anderson, M. A. E., Basu, S., Leftwich, P. T. & Alphey, L., 7 Jul 2023, biorxiv.

  Research output: Working paper › Preprint

  Open Access

• Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti

  Carabajal Paladino, L. Z., Wilson, R., Tng, P. Y. L., Dhokiya, V., Keen, E., Cuber, P., Larner, W., Rooney, S., Nicholls, M., Uglow, A., Williams, L., Anderson, M. A. E., Basu, S., Leftwich, P. T. & Alphey, L., 22 Sep 2023, In: Frontiers in Bioengineering and Biotechnology. 11, 1254863.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  1 Citation (Scopus)

  6 Downloads (Pure)
• 2022

  A multiplexed, confinable CRISPR/Cas9 gene drive propagates in caged Aedes aegypti populations

  Anderson, M. A. E., Gonzalez, E., Edgington, M. P., Ang, J. X. D., Purusothaman, D-K., Shackleford, L., Nevard, K., Verkuijl, S. A. N., Harvey-Samuel, T., Leftwich, P. T., Esvelt, K. & Alphey, L., 12 Aug 2022, biorxiv.

  Research output: Working paper › Preprint

  Open Access

• Characterisation of the symbionts in the Mediterranean fruit fly gut

  Darrington, M., Leftwich, P. T., Holmes, N. A., Friend, L. A., Clarke, N. V. E., Worsley, S. F., Margaritopolous, J. T., Hogenhout, S. A., Hutchings, M. I. & Chapman, T., 21 Apr 2022, In: Microbial Genomics. 8, 4, 000801.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  5 Citations (Scopus)

  12 Downloads (Pure)

• Drosophila Genetics Practicals - Independent Assortment, Sex Linked inheritance, Two/Three-point linkage

  Leftwich, P. T., 6 Dec 2022, Zenodo.

  Research output: Other contribution

  Open Access

• Manipulating insect sex determination pathways for genetic pest management: Opportunities and challenges

  Siddall, A., Harvey-Samuel, T., Chapman, T. & Leftwich, P. T., 28 Jun 2022, In: Frontiers in Bioengineering and Biotechnology. 10, 867851.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  9 Citations (Scopus)

  21 Downloads (Pure)

• Toward a CRISPR-Cas9-based gene drive in the diamondback moth Plutella xylostella

  Xu, X., Harvey-Samuel, T., Siddiqui, H. A., Ang, J. X. D., Anderson, M. E., Reitmayer, C. M., Lovett, E., Leftwich, P. T., You, M. & Alphey, L., Apr 2022, In: The CRISPR Journal. 5, 2, p. 224-236 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  25 Citations (Scopus)

  27 Downloads (Pure)
• 2021

  Characterisation of the symbionts in the Mediterranean fruitfly gut

  Darrington, M., Leftwich, P. T., Holmes, N. A., Friend, L. A., Clarke, N. V. E., Worsley, S. F., Margaritopoulos, J. T., Hogenhout, S. A., Hutchings, M. I. & Chapman, T., 10 Jun 2021

  Research output: Other contribution

• Engineered expression of the invertebrate‐specific scorpion toxin AaHIT reduces adult longevity and female fecundity in the diamondback moth Plutella xylostella

  Harvey‐Samuel, T. D., Xu, X., Lovett, E., Dafa'alla, T., Walker, A., Norman, V. C., Carter, R., Teal, J., Akilan, L., Leftwich, P. T., Reitmayer, C. M., Siddiqui, H. A. & Alphey, L., Jul 2021, In: Pest Management Science. 77, 7, p. 3154-3164 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  6 Citations (Scopus)

  41 Downloads (Pure)

• Genetic pest management and the background genetics of release strains

  Leftwich, P. T., Spurgin, L. G., Harvey-Samuel, T., Thomas, C. J. E., Paladino, L. C., Edgington, M. P. & Alphey, L., 15 Feb 2021, In: Philosophical Transactions of the Royal Society B: Biological Sciences. 376, 1818, 20190805.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  18 Citations (Scopus)

  13 Downloads (Pure)

• The Bioinformatics Virtual Coordination Network: An open-source and interactive learning environment

  Tully, B. J., Buongiorno, J., Cohen, A. B., Cram, J. A., Garber, A. I., Hu, S. K., Krinos, A. I., Leftwich, P. T., Marshall, A. J., Sieradzki, E. T., Speth, D. R., Suter, E. A., Trivedi, C. B., Valentin-Alvarado, L. E., Weissman, J. L. & BVCN Instructor Consortium, 14 Oct 2021, In: Frontiers in Education. 6, 711618.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  2 Citations (Scopus)

  6 Downloads (Pure)

• Towards CRISPR/Cas9-based gene drive in the diamondback moth Plutella xylostella

  Xu, X., Harvey-Samuel, T., Siddiqui, H. A., Ang, J., Anderson, M. E., Reitmayer, C., Lovett, E., Leftwich, P. T., You, M. & Alphey, L., 5 Oct 2021, biorxiv.

  Research output: Working paper

  Open Access
• 2020

  Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression

  Tng, P. Y. L., Paladino, L. C., Verkuijl, S. A. N., Purcell, J., Merits, A., Leftwich, P. T., Fragkoudis, R., Noad, R. & Alphey, L., Dec 2020, In: Communications Biology. 3, 413.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  21 Citations (Scopus)

  19 Downloads (Pure)

• Engineered expression of the invertebrate-specific scorpion toxin AaHIT reduces adult longevity and female fecundity in the diamondback moth Plutella xylostella

  Harvey-Samuel, T., Xu, X., Lovett, E., Dafa'alla, T., Walker, A. S., Norman, V. C., Carter, R., Teal, J., Akilan, L., Leftwich, P. T. & Alphey, L., 11 Sep 2020, biorxiv.

  Research output: Other contribution

• Expanding the CRISPR toolbox in Culicine mosquitoes: in vitro validation of Pol III promoters

  Anderson, M., Purcell, J., Verkuijl, S., Norman, V., Leftwich, P., Harvey-Samuel, T. & Alphey, L., 20 Mar 2020, In: ACS Synthetic Biology. 9, 3, p. 678-681 4 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  20 Citations (Scopus)

  20 Downloads (Pure)

• Transmission efficiency drives host–microbe associations

  Leftwich, P. T., Edgington, M. P. & Chapman, T., 9 Sep 2020, In: Proceedings of the Royal Society B: Biological Sciences. 287, 1934, 20200820.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  17 Downloads (Pure)
• 2019

  Contribution of maternal effects to dietary selection in Mediterranean fruit flies

  Leftwich, P. T., Nash, W. J., Friend, L. A. & Chapman, T., Feb 2019, In: Evolution. 73, 2, p. 278-292

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  3 Citations (Scopus)

  36 Downloads (Pure)

• Genetic frontiers for conservation: An assessment of synthetic biology and biodiversity conservation

  Redford, K. (ed.), Brooks, T. (ed.), Macfarlane, N. (ed.), Adams, J. (ed.), Alphey, L., Bennet, E., Delborne, J., Eggermont, H., Esvelt, K., Kingirl, A., Kokotovich, A., Kolodziejczyk, B., Kuiken, T., Mead, A., Oliva, M., Perello, E., Slobodian, L., Thizy, D., Tompkins, D., Winter, G. & 14 others, Campbell, K., Elsensohn, J., Holmes, N., Farmer, C., Keitt, B., Leftwich, P., Maloney, T., Masiga, D., Newhouse, A., Novak, B., Phelan, R., Powell, W., Rollins-Smith, L. & van Oppen, M., 2019, IUCN.

  Research output: Book/Report › Commissioned report

  Open Access

  File
  145 Downloads (Pure)
• 2018

  Diet, Gut Microbes and Host Mate Choice: Understanding the significance of microbiome effects on host mate choice requires a case by case evaluation

  Leftwich, P., Hutchings, M. & Chapman, T., Dec 2018, In: BioEssays. 40, 12, 1800053.

  Research output: Contribution to journal › Comment/debate › peer-review

  Open Access

  File
  8 Citations (Scopus)

  65 Downloads (Pure)

• Maths Skills for A-level Biology

  Penny, J. & Leftwich, P., 6 Sep 2018, 2nd ed. Oxford University Press. 136 p.

  Research output: Book/Report › Book

• Recent advances in threshold-dependent gene drives for mosquitoes

  Leftwich, P. T., Edgington, M. P., Harvey-Samuel, T., Carabajal Paladino, L. Z., Norman, V. C. & Alphey, L., 19 Oct 2018, In: Biochemical Society Transactions. 46, 5, p. 1203-1212 10 p.

  Research output: Contribution to journal › Review article › peer-review

  Open Access

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  30 Citations (Scopus)

  25 Downloads (Pure)

• Reply to Obadia et al.: Effect of methyl paraben on host–microbiota interactions in Drosophila melanogaster

  Leftwich, P. T., Clarke, N. VE., Hutchings, M. I. & Chapman, T., 15 May 2018, In: Proceedings of the National Academy of Sciences of the United States of America (PNAS). 115, 20, p. E4549-E4550 2 p.

  Research output: Contribution to journal › Comment/debate › peer-review

  Open Access

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  9 Citations (Scopus)

  26 Downloads (Pure)

• Reply to Rosenberg et al: Diet, gut bacteria and assortative mating in Drosophila melanogaster

  Leftwich, P. T., Clarke, N. VE., Hutchings, M. I. & Chapman, T., 6 Mar 2018, In: Proceedings of the National Academy of Sciences of the United States of America (PNAS). 115, 10, p. E2154-E2155 2 p.

  Research output: Contribution to journal › Comment/debate › peer-review
  8 Citations (Scopus)

• Testing for assortative mating by diet in Drosophila melanogaster

  Leftwich, P. T. & Chapman, T., 20 Oct 2018, In: BIO-Protocol. 8, 20, e3057.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  16 Downloads (Pure)
• 2017

  Adaptation to divergent larval diets in the medfly, Ceratitis capitata

  Leftwich, P. T., Nash, W. J., Friend, L. A. & Chapman, T., Feb 2017, In: Evolution. 71, 2, p. 289–303

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  18 Citations (Scopus)

  17 Downloads (Pure)

• Gut microbiomes and reproductive isolation in Drosophila

  Leftwich, P., Clarke, N., Hutchings, M. & Chapman, T., 28 Nov 2017, In: Proceedings of the National Academy of Sciences of the United States of America. 114, 48, p. 12767-12772 6 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  62 Citations (Scopus)

  22 Downloads (Pure)

• Vertically transmitted rhabdoviruses are found across three insect families and have dynamic interactions with their hosts

  Longdon, B., Day, J. P., Schulz, N., Leftwich, P. T., de Jong, M. A., Breuker, C. J., Gibbs, M., Obbard, D. J., Wilfert, L., Smith, S. C. L., McGonigle, J. E., Houslay, T. M., Wright, L. I., Livraghi, L., Evans, L. C., Friend, L. A., Chapman, T., Vontas, J., Kambouraki, N. & Jiggins, F. M., 25 Jan 2017, In: Proceedings of the Royal Society B: Biological Sciences. 284, 1847, 20162381.

  Research output: Contribution to journal › Article › peer-review

  Open Access
  31 Citations (Scopus)
• 2016

  Evolutionary biology and genetic techniques for insect control

  Leftwich, P., Bolton, M. & Chapman, T., Jan 2016, In: Evolutionary Applications. 9, 1, p. 212-230 19 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  70 Citations (Scopus)

  12 Downloads (Pure)
• 2014

  Genetic elimination of field-cage populations of Mediterranean fruit flies

  Leftwich, P. T., Koukidou, M., Rempoulakis, P., Gong, H-F., Zacharopoulou, A., Fu, G., Chapman, T., Economopoulos, A., Vontas, J. & Alphey, L., 7 Oct 2014, In: Proceedings of the Royal Society B: Biological Sciences. 281, 1792, 20141372.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  14 Downloads (Pure)
• 2012

  Variation in adult sex ratio alters the association between courtship, mating frequency and paternity in the lek-forming fruitfly Ceratitis capitata: Sex ratio and male reproductive success in the Mediterranean Fruitfly

  Leftwich, P. T., Edward, D. A., Alphey, L., Gage, M. J. G. & Chapman, T., 1 Sep 2012, In: Journal of Evolutionary Biology. 25, 9, p. 1732-1740

  Research output: Contribution to journal › Article › peer-review
  27 Citations (Scopus)