Stephen Bornemann

Search results

• 2022

  Pseudomonas syringae addresses distinct environmental challenges during plant infection through the coordinated deployment of polysaccharides

  Krishna, P. S., Woodcock, S. D., Pfeilmeier, S., Bornemann, S., Zipfel, C. & Malone, J. G., 5 Apr 2022, In: Journal of Experimental Botany. 73, 7, p. 2206–2221 16 p.

  Research output: Contribution to journal › Article › peer-review

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  9 Citations (Scopus)

  16 Downloads (Pure)
• 2021

  A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of α-maltose-1-phosphate

  Syson, K., Batey, S. F. D., Schindler, S., Kalscheuer, R. & Bornemann, S., Feb 2021, In: Biochimica Et Biophysica Acta-General Subjects. 1865, 2, 129783.

  Research output: Contribution to journal › Article › peer-review

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  2 Citations (Scopus)

  9 Downloads (Pure)

• Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa

  Woodcock, S. D., Syson, K., Little, R. H., Ward, D., Sifouna, D., Brown, J. K. M., Bornemann, S. & Malone, J. G., 19 Apr 2021, In: PLoS Genetics. 17, 4, e1009524.

  Research output: Contribution to journal › Article › peer-review

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  23 Citations (SciVal)

  11 Downloads (Pure)
• 2020

  Structure of the Mycobacterium smegmatis alpha-maltose-1-phosphate synthase GlgM

  Syson, K., Stevenson, C. E. M., Lawson, D. M. & Bornemann, S., 1 Apr 2020, In: Acta crystallographica section f-Structural biology communications. 76, p. 175-181 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  5 Citations (Scopus)

  15 Downloads (Pure)
• 2017

  The production and utilization of GDP-glucose in the biosynthesis of trehalose 6-phosphate by Streptomyces venezuelae

  Asencion Diez, M. D., Miah, F., Stevenson, C. E. M., Lawson, D. M., Iglesias, A. A. & Bornemann, S., 20 Jan 2017, In: Journal of Biological Chemistry. 292, 3, p. 945-954 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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  11 Citations (Scopus)

  10 Downloads (Pure)
• 2016

  alpha-Glucan biosynthesis and the GlgE pathway in Mycobacterium tuberculosis

  Bornemann, S., 9 Feb 2016, In: Biochemical Society Transactions. 44, 1, p. 68-73 6 p.

  Research output: Contribution to journal › Review article › peer-review
  20 Citations (Scopus)

• Assembly of α-glucan by GlgE and GlgB in mycobacteria and streptomycetes

  Rashid, A. M., Batey, S. F. D., Syson, K., Koliwer-Brandl, H., Miah, F., Barclay, J. E., Findlay, K. C., Nartowski, K. P., Khimyak, Y. Z., Kalscheuer, R. & Bornemann, S., 14 Jun 2016, In: Biochemistry. 55, 23, p. 3270-3284 15 p.

  Research output: Contribution to journal › Article › peer-review

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  32 Citations (SciVal)

  17 Downloads (Pure)

• Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of alpha-maltose 1-phosphate

  Miah, F., Bibb, M. J., Barclay, J. E., Findlay, K. C. & Bornemann, S., 1 Jul 2016, In: Microbiology. 162, p. 1208-1219 12 p.

  Research output: Contribution to journal › Article › peer-review

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  10 Citations (Scopus)

  13 Downloads (Pure)

• Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor Maltosyltransferase GlgE

  Syson, K., Stevenson, C. E. M., Miah, F., Barclay, J. E., Tang, M., Gorelik, A., Rashid, A. M., Lawson, D. M. & Bornemann, S., 7 Oct 2016, In: Journal of Biological Chemistry. 291, 41, p. 21531-21540 10 p.

  Research output: Contribution to journal › Article › peer-review

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  10 Citations (Scopus)

  15 Downloads (Pure)

• Metabolic network for the biosynthesis of intra- and extracellular alpha-glucans required for virulence of Mycobacterium tuberculosis

  Koliwer-Brandl, H., Syson, K., van de Weerd, R., Chandra, G., Appelmelk, B., Alber, M., Ioerger, T. R., Jacobs, W. R., Geurtsen, J., Bornemann, S. & Kalscheuer, R., 11 Aug 2016, In: PLoS Pathogens. 12, 8, 1005768.

  Research output: Contribution to journal › Article › peer-review

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  41 Citations (Scopus)

  12 Downloads (Pure)

• Trehalose-6-phosphate-mediated toxicity determines essentiality of OtsB2 in Mycobacterium tuberculosis in vitro and in mice

  Korte, J., Alber, M., Trujillo, C. M., Syson, K., Koliwer-Brandl, H., Deenen, R., Köhrer, K., DeJesus, M. A., Hartman, T., Jacobs, W. R., Bornemann, S., Ioerger, T. R., Ehrt, S. & Kalscheuer, R., 9 Dec 2016, In: PLoS Pathogens. 12, 12, e1006043.

  Research output: Contribution to journal › Article › peer-review

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  31 Citations (SciVal)

  10 Downloads (Pure)
• 2015

  Allosteric regulation of the partitioning of glucose-1-phosphate between glycogen and trehalose biosynthesis in Mycobacterium tuberculosis

  Asención Diez, M. D., Demonte, A. M., Syson, K., Arias, D. G., Gorelik, A., Guerrero, S. A., Bornemann, S. & Iglesias, A. A., Jan 2015, In: Biochimica Et Biophysica Acta-General Subjects. 1850, 1, p. 13-21 9 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access
  22 Citations (Scopus)
• 2014

  Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds alpha-maltose 1-phosphate and forms a maltosyl-enzyme intermediate

  Syson, K., Stevenson, C. E. M., Rashid, A. M., Saalbach, G., Tang, M., Tuukkanen, A., Svergun, D. I., Withers, S. G., Lawson, D. M. & Bornemann, S., 22 Apr 2014, In: Biochemistry. 53, 15, p. 2494-2504 11 p.

  Research output: Contribution to journal › Article › peer-review
  30 Citations (Scopus)

• Sugar-coated sensor chip and nanoparticle surfaces for the in vitro enzymatic synthesis of starch-like materials

  O'Neill, E. C., Rashid, A. M., Stevenson, C. E. M., Hetru, A. C., Gunning, A. P., Rejzek, M., Nepogodiev, S. A., Bornemann, S., Lawson, D. M. & Field, R. A., Jan 2014, In: Chemical Science. 5, 1, p. 341-350 10 p.

  Research output: Contribution to journal › Article › peer-review
  28 Citations (Scopus)
• 2013

  Calcium/Calmodulin-dependent protein kinase is negatively and positively regulated by calcium, providing a mechanism for decoding calcium responses during symbiosis signaling

  Miller, J. B., Pratap, A., Miyahara, A., Zhou, L., Bornemann, S., Morris, R. J. & Oldroyd, G. E. D., Dec 2013, In: The Plant Cell. 25, 12, p. 5053-5066 14 p.

  Research output: Contribution to journal › Article › peer-review
  116 Citations (Scopus)

• Flux through trehalose synthase flows from trehalose to the alpha anomer of maltose in mycobacteria

  Miah, F., Koliwer-Brandl, H., Rejzek, M., Field, R. A., Kalscheuer, R. & Bornemann, S., 18 Apr 2013, In: Chemistry and Biology. 20, 4, p. 487-493 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access
  40 Citations (Scopus)

• Mycobacterium tuberculosis maltosyltransferase GlgE, a genetically validated antituberculosis target, is negatively regulated by Ser/Thr phosphorylation

  Leiba, J., Syson, K., Baronian, G., Zanella-Cleon, I., Kalscheuer, R., Kremer, L., Bornemann, S. & Molle, V., 7 Jun 2013, In: Journal of Biological Chemistry. 288, 23, p. 16546-16556 11 p.

  Research output: Contribution to journal › Article › peer-review
  34 Citations (Scopus)
• 2012

  Calcium ion binding properties of medicago truncatula calcium/calmodulin- dependent protein kinase

  Swainsbury, D. J. K., Zhou, L., Oldroyd, G. E. D. & Bornemann, S., 4 Sept 2012, In: Biochemistry. 51, 35, p. 6897-6907 11 p.

  Research output: Contribution to journal › Article › peer-review
  18 Citations (Scopus)
• 2011

  Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target

  Syson, K., Stevenson, C. E. M., Rejzek, M., Fairhurst, S. A., Nair, A., Bruton, C. J., Field, R. A., Chater, K. F., Lawson, D. M. & Bornemann, S., 4 Nov 2011, In: Journal of Biological Chemistry. 286, 44, p. 38298-38310 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access
  52 Citations (Scopus)
• 2010

  Detection of enzyme-catalyzed polysaccharide synthesis on surfaces

  Clé, C., Martin, C., Field, R. A., Kuzmič, P. & Bornemann, S., 2010, In: Biocatalysis and Biotransformation. 28, 1, p. 64-71 8 p.

  Research output: Contribution to journal › Review article › peer-review
  4 Citations (Scopus)
• 2009

  pH-Dependent structures of the manganese binding sites in oxalate decarboxylase as revealed by high-field electron paramagnetic resonance

  Tabares, L. C., Gätjens, J., Hureau, C., Burrell, M. R., Bowater, L., Pecoraro, V. L., Bornemann, S. & Un, S., 2009, In: The Journal of Physical Chemistry B. 113, 26, p. 9016-9025 10 p.

  Research output: Contribution to journal › Article › peer-review
  30 Citations (Scopus)
• 2008

  Detection of transglucosidase-catalyzed polysaccharide synthesis on a surface in real time using surface plasmon resonance spectroscopy

  Clé, C., Gunning, A. P., Syson, K., Bowater, L., Field, R. A. & Bornemann, S., 2008, In: Journal of the American Chemical Society. 130, 46, p. 15234-15235 2 p.

  Research output: Contribution to journal › Article
  18 Citations (Scopus)
• 2007

  Oxalate decarboxylase and oxalate oxidase activities can be interchanged with a specificity switch of up to 282 000 by mutating an active site lid

  Burrell, M. R., Just, V. J., Bowater, L., Fairhurst, S. A., Requena, L., Lawson, D. M. & Bornemann, S., 2007, In: Biochemistry. 46, 43, p. 12327-12336 10 p.

  Research output: Contribution to journal › Article
  46 Citations (Scopus)

• The identity of the active site of oxalate decarboxylase and the importance of the stability of active-site lid conformations

  Burrell, M. R., Just, V. J., Bowater, L., McRobbie, I., Stevenson, C. E. M., Lawson, D. M. & Bornemann, S., 1 Nov 2007, In: Biochemical Journal. 407, 3, p. 397-406 10 p.

  Research output: Contribution to journal › Article
  30 Citations (Scopus)
• 2005

  Cloning and sequencing of two Ceriporiopsis subvermispora bicupin oxalate oxidase allelic isoforms: Implications for the reaction specificity of oxalate oxidases and decarboxylases

  Escutia, M. R., Bowater, L., Edwards, A., Bottrill, A. R., Burrell, M. R., Polanco, R., Vicuña, R. & Bornemann, S., 2005, In: Applied and Environmental Microbiology. 71, 7, p. 3608-3616 9 p.

  Research output: Contribution to journal › Article
  51 Citations (Scopus)
• 2004

  A closed conformation of Bacillus subtilis oxalate decarboxylase OxdC provides evidence for the true identity of the active site

  Just, V. J., Stephenson, C. E., Bowater, L., Tanner, A., Lawson, D. M. & Bornemann, S., 2004, In: Journal of Biological Chemistry. 279, 19, p. 19867-19874 8 p.

  Research output: Contribution to journal › Article

  Open Access
  84 Citations (Scopus)

• Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase

  Bowater, L., Fairhurst, S. A., Just, V. J. & Bornemann, S., 2004, In: FEBS Letters. 557, 1-3, p. 45-48 4 p.

  Research output: Contribution to journal › Article
  68 Citations (Scopus)

• SAD at home: solving the structure of oxalate decarboxylase with the anomalous signal from manganese using X-ray data collected on a home source

  Stevenson, C. E. M., Tanner, A., Bowater, L., Bornemann, S. & Lawson, D. M., 2004, In: Acta Crystallographica Section D: Biological Crystallography. 60, 12, p. 2403-2406 4 p.

  Research output: Contribution to journal › Article
  7 Citations (Scopus)