TY - JOUR
T1 - β-1,2-Oligomannan phosphorylase-mediated synthesis of potential oligosaccharide vaccine candidates
AU - Ahmadipour, Sanaz
AU - Winsbury, Rebecca
AU - Köhler, Dominic
AU - Pergolizzi, Giulia
AU - Nepogodiev, Sergey A.
AU - Chessa, Simona
AU - Dedola, Simone
AU - Wang, Meng
AU - Voglmeir, Josef
AU - Field, Robert A.
N1 - Data availability statement: No data was used for the research described in the article.
Funding Information: Innovate UK in the form of an Innovation Scholar Secondment to SA (UKRI, 76242: Sugars, Enzymes and Diagnostics (SEAD)). Work at the John Innes Centre was supported by the UK BBSRC Institute Strategic Program on Molecules from Nature -Products and Pathways [ BBS/E/J/000PR9790 ] and the John Innes Foundation.
PY - 2023/6
Y1 - 2023/6
N2 - β-(1,2)-Mannan antigens incorporated into vaccines candidates for immunization studies, showed that antibodies raised against β-(1,2)-mannotriose antigens can protect against disseminated candidiasis. Until recently, β-(1,2)- mannans could only be obtained by isolation from microbial cultures, or by lengthy synthetic strategies involving protecting group manipulation. The discovery of two β-(1,2)-mannoside phosphorylases, Teth514_1788 and Teth514_1789, allowed efficient access to these compounds. In this work, Teth514_1788 was utilised to generate β-(1,2)-mannan antigens, tri- and tetra-saccharides, decorated with a conjugation tether at the reducing end, suitable to be incorporated on a carrier en-route to novel vaccine candidates, illustrated here by conjugation of the trisaccharide to BSA.
AB - β-(1,2)-Mannan antigens incorporated into vaccines candidates for immunization studies, showed that antibodies raised against β-(1,2)-mannotriose antigens can protect against disseminated candidiasis. Until recently, β-(1,2)- mannans could only be obtained by isolation from microbial cultures, or by lengthy synthetic strategies involving protecting group manipulation. The discovery of two β-(1,2)-mannoside phosphorylases, Teth514_1788 and Teth514_1789, allowed efficient access to these compounds. In this work, Teth514_1788 was utilised to generate β-(1,2)-mannan antigens, tri- and tetra-saccharides, decorated with a conjugation tether at the reducing end, suitable to be incorporated on a carrier en-route to novel vaccine candidates, illustrated here by conjugation of the trisaccharide to BSA.
KW - Candida pathogen
KW - Click chemistry
KW - Glycoconjugate vaccines
KW - β-(1,2)-Mannan antigen
KW - β-(1,2)-Oligomannan phosphorylase
UR - https://www.scopus.com/pages/publications/85153076393
U2 - 10.1016/j.carres.2023.108807
DO - 10.1016/j.carres.2023.108807
M3 - Article
C2 - 37094534
AN - SCOPUS:85153076393
SN - 0008-6215
VL - 528
JO - Carbohydrate Research
JF - Carbohydrate Research
M1 - 108807
ER -
SDG 3 Good Health and Well-being