1,2-diacylglycerol kinase of human erythrocyte membranes. Assay with endogenously generated substrate

D ALLAN, P THOMAS, S GATT

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Abstract

1,2-Diacylglycerol kinase activity was measured in human erythrocyte membranes using an assay procedure in which the substrate was generated endogenously, either by treatment with a bacterial phospholipase C or by incubation with Ca24, which activates a membrane-bound polyphosphoinositide phosphodiesterase. The properties of 1,2-diacylglycerol kinase were broadly similar to those described previously, except that in the present work maximum activities were higher and there was evidence for a double pH optimum.
Original languageEnglish
Pages (from-to)669–672
Number of pages4
JournalBiochemical Journal
Volume191
Issue number2
DOIs
Publication statusPublished - 1 Nov 1980

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