4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues

David Baddeley, David Crossman, Sabrina Rossberger, Juliette E Cheyne, Johanna M Montgomery, Isuru D Jayasinghe, Christoph Cremer, Mark B Cannell, Christian Soeller

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the same sample.
Original languageEnglish
Article numbere20645
JournalPLoS One
Volume6
Issue number5
DOIs
Publication statusPublished - 2011

Keywords

  • Animals
  • Cells, Cultured
  • Cyclic AMP
  • Humans
  • Microscopy, Fluorescence
  • Models, Theoretical
  • Myocytes, Cardiac
  • Rats
  • Succinimides

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