A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

Heleen M de Vogel-van den Bosch, Nicole J W de Wit, Guido J E J Hooiveld, Hanneke Vermeulen, Jelske N van der Veen, Sander M Houten, Folkert Kuipers, Michael Müller, Roelof van der Meer

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P
Original languageEnglish
Pages (from-to)G1171-1180
Number of pages10
JournalAJP: Gastrointestinal and Liver Physiology
Volume294
Issue number5
DOIs
Publication statusPublished - May 2008

Keywords

  • ATP Binding Cassette Transporter 1
  • ATP-Binding Cassette Transporters
  • Animals
  • Bile Acids and Salts
  • Cholesterol
  • DNA-Binding Proteins
  • Dietary Fats
  • Epithelial Cells
  • Feces
  • Gene Expression Regulation
  • Hydroxycholesterols
  • Hydroxymethylglutaryl CoA Reductases
  • Intestinal Absorption
  • Intestine, Small
  • Lipoproteins
  • Male
  • Membrane Transport Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Models, Biological
  • Oligonucleotide Array Sequence Analysis
  • Orphan Nuclear Receptors
  • Receptors, Cytoplasmic and Nuclear
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triglycerides

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