A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

Heleen M de Vogel-van den Bosch, Nicole J W de Wit, Guido J E J Hooiveld, Hanneke Vermeulen, Jelske N van der Veen, Sander M Houten, Folkert Kuipers, Michael Müller, Roelof van der Meer

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51 Citations (Scopus)


Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P
Original languageEnglish
Pages (from-to)G1171-1180
Number of pages10
JournalAJP: Gastrointestinal and Liver Physiology
Issue number5
Publication statusPublished - May 2008


  • ATP Binding Cassette Transporter 1
  • ATP-Binding Cassette Transporters
  • Animals
  • Bile Acids and Salts
  • Cholesterol
  • DNA-Binding Proteins
  • Dietary Fats
  • Epithelial Cells
  • Feces
  • Gene Expression Regulation
  • Hydroxycholesterols
  • Hydroxymethylglutaryl CoA Reductases
  • Intestinal Absorption
  • Intestine, Small
  • Lipoproteins
  • Male
  • Membrane Transport Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Models, Biological
  • Oligonucleotide Array Sequence Analysis
  • Orphan Nuclear Receptors
  • Receptors, Cytoplasmic and Nuclear
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triglycerides

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