A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

Heleen M de Vogel-van den Bosch; Nicole J W de Wit; Guido J E J Hooiveld; Hanneke Vermeulen; Jelske N van der Veen; Sander M Houten; Folkert Kuipers; Michael Müller; Roelof van der Meer

doi:10.1152/ajpgi.00360.2007

A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

Heleen M de Vogel-van den Bosch, Nicole J W de Wit, Guido J E J Hooiveld, Hanneke Vermeulen, Jelske N van der Veen, Sander M Houten, Folkert Kuipers, Michael Müller, Roelof van der Meer

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P

Original language	English
Pages (from-to)	G1171-1180
Number of pages	10
Journal	AJP: Gastrointestinal and Liver Physiology
Volume	294
Issue number	5
DOIs	https://doi.org/10.1152/ajpgi.00360.2007
Publication status	Published - May 2008

Keywords

ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters
Animals
Bile Acids and Salts
Cholesterol
DNA-Binding Proteins
Dietary Fats
Epithelial Cells
Feces
Gene Expression Regulation
Hydroxycholesterols
Hydroxymethylglutaryl CoA Reductases
Intestinal Absorption
Intestine, Small
Lipoproteins
Male
Membrane Transport Proteins
Mice
Mice, Inbred C57BL
Mice, Knockout
Models, Biological
Oligonucleotide Array Sequence Analysis
Orphan Nuclear Receptors
Receptors, Cytoplasmic and Nuclear
Reverse Transcriptase Polymerase Chain Reaction
Triglycerides

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10.1152/ajpgi.00360.2007

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de Vogel-van den Bosch, H. M., de Wit, N. J. W., Hooiveld, G. J. E. J., Vermeulen, H., van der Veen, J. N., Houten, S. M., Kuipers, F., Müller, M., & van der Meer, R. (2008). A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine. AJP: Gastrointestinal and Liver Physiology, 294(5), G1171-1180. https://doi.org/10.1152/ajpgi.00360.2007

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title = "A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine",

abstract = "Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P ",

keywords = "ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters, Animals, Bile Acids and Salts, Cholesterol, DNA-Binding Proteins, Dietary Fats, Epithelial Cells, Feces, Gene Expression Regulation, Hydroxycholesterols, Hydroxymethylglutaryl CoA Reductases, Intestinal Absorption, Intestine, Small, Lipoproteins, Male, Membrane Transport Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Biological, Oligonucleotide Array Sequence Analysis, Orphan Nuclear Receptors, Receptors, Cytoplasmic and Nuclear, Reverse Transcriptase Polymerase Chain Reaction, Triglycerides",

author = "{de Vogel-van den Bosch}, {Heleen M} and {de Wit}, {Nicole J W} and Hooiveld, {Guido J E J} and Hanneke Vermeulen and {van der Veen}, {Jelske N} and Houten, {Sander M} and Folkert Kuipers and Michael M{\"u}ller and {van der Meer}, Roelof",

year = "2008",

month = may,

doi = "10.1152/ajpgi.00360.2007",

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de Vogel-van den Bosch, HM, de Wit, NJW, Hooiveld, GJEJ, Vermeulen, H, van der Veen, JN, Houten, SM, Kuipers, F, Müller, M & van der Meer, R 2008, 'A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine', AJP: Gastrointestinal and Liver Physiology, vol. 294, no. 5, pp. G1171-1180. https://doi.org/10.1152/ajpgi.00360.2007

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T1 - A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

AU - de Vogel-van den Bosch, Heleen M

AU - de Wit, Nicole J W

AU - Hooiveld, Guido J E J

AU - Vermeulen, Hanneke

AU - van der Veen, Jelske N

AU - Houten, Sander M

AU - Kuipers, Folkert

AU - Müller, Michael

AU - van der Meer, Roelof

PY - 2008/5

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N2 - Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P

AB - Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P

KW - ATP Binding Cassette Transporter 1

KW - ATP-Binding Cassette Transporters

KW - Animals

KW - Bile Acids and Salts

KW - Cholesterol

KW - DNA-Binding Proteins

KW - Dietary Fats

KW - Epithelial Cells

KW - Feces

KW - Gene Expression Regulation

KW - Hydroxycholesterols

KW - Hydroxymethylglutaryl CoA Reductases

KW - Intestinal Absorption

KW - Intestine, Small

KW - Lipoproteins

KW - Male

KW - Membrane Transport Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Models, Biological

KW - Oligonucleotide Array Sequence Analysis

KW - Orphan Nuclear Receptors

KW - Receptors, Cytoplasmic and Nuclear

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Triglycerides

U2 - 10.1152/ajpgi.00360.2007

DO - 10.1152/ajpgi.00360.2007

M3 - Article

C2 - 18356535

SN - 0193-1857

VL - 294

SP - G1171-1180

JO - AJP: Gastrointestinal and Liver Physiology

JF - AJP: Gastrointestinal and Liver Physiology

IS - 5

ER -