A communal catalogue reveals Earth’s multiscale microbial diversity

Luke Thompson, Jon Sanders, Daniel McDonald, Amnon Amir, Joshua Ladau, Kenneth Locey, Robert Prill, Anupriya Tripathi, Sean Gibbons, Gail Ackermann, Jose Navas-Molina, Stefan Janssen, Evguenia Kopylova, Yoshiki Vázquez-Baeza, Antonio González, James Morton, Siavash Mirarab, Zhenjiang Zech Xu, Lingjing Jiang, Mohamed F. HaroonJad Kanbar, Qiyun Zhu, Se Jin Song, Tomasz Kosciolek, Nicholas Bokulich, Joshua Lefler, Colin Brislawn, Gregory Humphrey, Sarah Owens, Jarrad Hampton-Marcell, Donna Berg-Lyons, Valerie McKenzie, Noah Fierer, Jed Fuhrman, Aaron Clauset, Rick Stevens, Ashley Shade, Katherine Pollard, Kelly Goodwin, Janet Jansson, Jack Gilbert, Robert Knight, The Earth Microbiome Project Consortium

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Abstract

Our growing awareness of the microbial world’s importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth’s microbial diversity.
Original languageEnglish
Pages (from-to)457–463
Number of pages7
JournalNature
Volume551
Early online date1 Nov 2017
DOIs
Publication statusPublished - 23 Nov 2017

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