A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

Nicholas Foote; Paul M. A. Gadsby; Robert A. Field; Colin Greenwood; Andrew J. Thomson

doi:10.1016/0014-5793(87)80085-6

A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

Nicholas Foote, Paul M. A. Gadsby, Robert A. Field, Colin Greenwood, Andrew J. Thomson

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6-50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P^.+].

Original language	English
Pages (from-to)	347-350
Number of pages	4
Journal	FEBS Letters
Volume	214
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(87)80085-6
Publication status	Published - 20 Apr 1987

Keywords

Compound II
Compound X
Horseradish peroxidase
Magnetic circular dichroism

Access to Document

10.1016/0014-5793(87)80085-6

Cite this

@article{0f77a1e8cd5243a6874dad91e7b66dbf,

title = "A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase",

abstract = "The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6-50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P.+].",

keywords = "Compound II, Compound X, Horseradish peroxidase, Magnetic circular dichroism",

author = "Nicholas Foote and Gadsby, {Paul M. A.} and Field, {Robert A.} and Colin Greenwood and Thomson, {Andrew J.}",

note = "Funding Information: A.J.T. and C.G. thank the SERC and the Royal Society for grants in support of this work.",

year = "1987",

month = apr,

day = "20",

doi = "10.1016/0014-5793(87)80085-6",

language = "English",

volume = "214",

pages = "347--350",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

AU - Foote, Nicholas

AU - Gadsby, Paul M. A.

AU - Field, Robert A.

AU - Greenwood, Colin

AU - Thomson, Andrew J.

N1 - Funding Information: A.J.T. and C.G. thank the SERC and the Royal Society for grants in support of this work.

PY - 1987/4/20

Y1 - 1987/4/20

N2 - The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6-50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P.+].

AB - The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6-50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P.+].

KW - Compound II

KW - Compound X

KW - Horseradish peroxidase

KW - Magnetic circular dichroism

UR - http://www.scopus.com/inward/record.url?scp=0023660340&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)80085-6

DO - 10.1016/0014-5793(87)80085-6

M3 - Article

C2 - 3569529

AN - SCOPUS:0023660340

SN - 0014-5793

VL - 214

SP - 347

EP - 350

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

Abstract

Keywords

Access to Document

Other files and links

Cite this