A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

Kamil Witek, Xiao Lin, Hari S. Karki, Florian Jupe, Agnieszka I. Witek, Burkhard Steuernagel, Remco Stam, Cock Van Oosterhout, Sebastian Fairhead, Robert Heal, Jonathan M. Cocker, Shivani Bhanvadia, William Barrett, Chih-Hang Wu, Hiroaki Adachi, Tianqiao Song, Sophien Kamoun, Vivianne G. A. A. Vleeshouwers, Laurence Tomlinson, Brande B. H. WulffJonathan D. G. Jones

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.
Original languageEnglish
Pages (from-to)198-208
Number of pages11
JournalNature Plants
Volume7
Issue number2
DOIs
Publication statusPublished - 11 Feb 2021

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