A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

Kamil Witek; Xiao Lin; Hari S. Karki; Florian Jupe; Agnieszka I. Witek; Burkhard Steuernagel; Remco Stam; Cock Van Oosterhout; Sebastian Fairhead; Robert Heal; Jonathan M. Cocker; Shivani Bhanvadia; William Barrett; Chih-Hang Wu; Hiroaki Adachi; Tianqiao Song; Sophien Kamoun; Vivianne G. A. A. Vleeshouwers; Laurence Tomlinson; Brande B. H. Wulff; Jonathan D. G. Jones

doi:10.1038/s41477-021-00854-9

A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

Kamil Witek, Xiao Lin, Hari S. Karki, Florian Jupe, Agnieszka I. Witek, Burkhard Steuernagel, Remco Stam, Cock Van Oosterhout, Sebastian Fairhead, Robert Heal, Jonathan M. Cocker, Shivani Bhanvadia, William Barrett, Chih-Hang Wu, Hiroaki Adachi, Tianqiao Song, Sophien Kamoun, Vivianne G. A. A. Vleeshouwers, Laurence Tomlinson, Brande B. H. WulffJonathan D. G. Jones

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Abstract

Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.

Original language	English
Pages (from-to)	198-208
Number of pages	11
Journal	Nature Plants
Volume	7
Issue number	2
DOIs	https://doi.org/10.1038/s41477-021-00854-9
Publication status	Published - 11 Feb 2021

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Witek, K., Lin, X., Karki, H. S., Jupe, F., Witek, A. I., Steuernagel, B., Stam, R., Van Oosterhout, C., Fairhead, S., Heal, R., Cocker, J. M., Bhanvadia, S., Barrett, W., Wu, C-H., Adachi, H., Song, T., Kamoun, S., Vleeshouwers, V. G. A. A., Tomlinson, L., ... Jones, J. D. G. (2021). A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector. Nature Plants, 7(2), 198-208. https://doi.org/10.1038/s41477-021-00854-9

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title = "A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector",

abstract = "Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.",

author = "Kamil Witek and Xiao Lin and Karki, {Hari S.} and Florian Jupe and Witek, {Agnieszka I.} and Burkhard Steuernagel and Remco Stam and {Van Oosterhout}, Cock and Sebastian Fairhead and Robert Heal and Cocker, {Jonathan M.} and Shivani Bhanvadia and William Barrett and Chih-Hang Wu and Hiroaki Adachi and Tianqiao Song and Sophien Kamoun and Vleeshouwers, {Vivianne G. A. A.} and Laurence Tomlinson and Wulff, {Brande B. H.} and Jones, {Jonathan D. G.}",

year = "2021",

month = feb,

day = "11",

doi = "10.1038/s41477-021-00854-9",

language = "English",

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Witek, K, Lin, X, Karki, HS, Jupe, F, Witek, AI, Steuernagel, B, Stam, R, Van Oosterhout, C, Fairhead, S, Heal, R, Cocker, JM, Bhanvadia, S, Barrett, W, Wu, C-H, Adachi, H, Song, T, Kamoun, S, Vleeshouwers, VGAA, Tomlinson, L, Wulff, BBH & Jones, JDG 2021, 'A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector', Nature Plants, vol. 7, no. 2, pp. 198-208. https://doi.org/10.1038/s41477-021-00854-9

TY - JOUR

T1 - A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

AU - Witek, Kamil

AU - Lin, Xiao

AU - Karki, Hari S.

AU - Jupe, Florian

AU - Witek, Agnieszka I.

AU - Steuernagel, Burkhard

AU - Stam, Remco

AU - Van Oosterhout, Cock

AU - Fairhead, Sebastian

AU - Heal, Robert

AU - Cocker, Jonathan M.

AU - Bhanvadia, Shivani

AU - Barrett, William

AU - Wu, Chih-Hang

AU - Adachi, Hiroaki

AU - Song, Tianqiao

AU - Kamoun, Sophien

AU - Vleeshouwers, Vivianne G. A. A.

AU - Tomlinson, Laurence

AU - Wulff, Brande B. H.

AU - Jones, Jonathan D. G.

PY - 2021/2/11

Y1 - 2021/2/11

N2 - Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.

AB - Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.

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U2 - 10.1038/s41477-021-00854-9

DO - 10.1038/s41477-021-00854-9

M3 - Article

VL - 7

SP - 198

EP - 208

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

IS - 2

ER -

A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

Abstract

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