Abstract
Ligand recognition by cell-surface receptors underlies development and immunity in both animals and plants. Modulating receptor signalling is critical for appropriate cellular responses but the mechanisms ensuring this are poorly understood. Here, we show that signalling by plant receptors for pathogen-associated molecular patterns (PAMPs) in immunity and CLAVATA3/EMBRYO SURROUNDING REGION-RELATED peptides (CLEp) in development uses a similar regulatory module. In the absence of ligand, signalling is dampened through association with specific type-2C protein phosphatases. Upon activation, PAMP and CLEp receptors phosphorylate divergent cytosolic kinases, which, in turn, phosphorylate the phosphatases, thereby promoting receptor signalling. Our work reveals a regulatory circuit shared between immune and developmental receptor signalling, which may have broader important implications for plant receptor kinase-mediated signalling in general.
| Original language | English |
|---|---|
| Pages (from-to) | 356-365 |
| Number of pages | 10 |
| Journal | Nature Plants |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2022 |
| Externally published | Yes |
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A conserved module regulates receptor kinase signalling in immunity and development. / DeFalco, Thomas A.; Anne, Pauline; James, Sean R. et al.
In: Nature Plants, Vol. 8, No. 4, 04.2022, p. 356-365.Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - A conserved module regulates receptor kinase signalling in immunity and development
AU - DeFalco, Thomas A.
AU - Anne, Pauline
AU - James, Sean R.
AU - Willoughby, Andrew C.
AU - Schwanke, Florian
AU - Johanndrees, Oliver
AU - Genolet, Yasmine
AU - Derbyshire, Paul
AU - Wang, Qian
AU - Rana, Surbhi
AU - Pullen, Anne-Marie
AU - Menke, Frank L. H.
AU - Zipfel, Cyril
AU - Hardtke, Christian S.
AU - Nimchuk, Zachary L.
N1 - Funding Information: This research was supported by the Gatsby Charitable Foundation (C.Z.), the University of Zürich (C.Z.), the European Research Council under the grant agreement nos. 309858 and 773153 (grants ‘PHOSPHOinnATE’ and ‘IMMUNO-PEPTALK’ to C.Z.), the Swiss National Science Foundation (grant agreement nos. 31003A_182625 to C.Z. and 310030B_185379 to C.S.H.), a National Institute of General Medical Sciences—Maximizing Investigators’ Research Award from the NIH (R35GM119614 to Z.L.N.), the National Science Foundation (IOS-1455607 to Z.L.N.), startup funds from Virginia Tech to Z.L.N. and a joint European Research Area Network for Coordinating Action in Plant Sciences (ERA-CAPS) grant (‘SICOPID’) from UK Research and Innovation (BB/S004734/1 to C.Z.) and National Science Foundation (IOS-1841917 to Z.L.N.), respectively. T.A.D. and P.A. were supported by the European Molecular Biology Organization (fellowships EMBO-LTF-100-2017 to T.A.D. and EMBO-LTF-480-2016 to P.A.). T.A.D. was further supported by the Natural Sciences and Engineering Council of Canada (fellowship PDF-532561-2019). P.A. and Y.G. were also supported by a Tremplin grant from the University of Lausanne. Funding Information: We thank J.-M. Zhou (CAS, Beijing) for kindly providing published rlck-vii mutants and P. Tarr (Caltech, USA) for the Myr-mTurquoise2 plasmid. The Nimchuk laboratory thanks T. D. Perdue, director of the University of North Carolina—Chapel Hill Genome Sciences Microscopy Core, for assistance with confocal imaging. The Zipfel group thanks all members for discussions and critical reading of the manuscript. This research was supported by the Gatsby Charitable Foundation (C.Z.), the University of Zürich (C.Z.), the European Research Council under the grant agreement nos. 309858 and 773153 (grants ‘PHOSPHOinnATE’ and ‘IMMUNO-PEPTALK’ to C.Z.), the Swiss National Science Foundation (grant agreement nos. 31003A_182625 to C.Z. and 310030B_185379 to C.S.H.), a National Institute of General Medical Sciences—Maximizing Investigators’ Research Award from the NIH (R35GM119614 to Z.L.N.), the National Science Foundation (IOS-1455607 to Z.L.N.), startup funds from Virginia Tech to Z.L.N. and a joint European Research Area Network for Coordinating Action in Plant Sciences (ERA-CAPS) grant (‘SICOPID’) from UK Research and Innovation (BB/S004734/1 to C.Z.) and National Science Foundation (IOS-1841917 to Z.L.N.), respectively. T.A.D. and P.A. were supported by the European Molecular Biology Organization (fellowships EMBO-LTF-100-2017 to T.A.D. and EMBO-LTF-480-2016 to P.A.). T.A.D. was further supported by the Natural Sciences and Engineering Council of Canada (fellowship PDF-532561-2019). P.A. and Y.G. were also supported by a Tremplin grant from the University of Lausanne.
PY - 2022/4
Y1 - 2022/4
N2 - Ligand recognition by cell-surface receptors underlies development and immunity in both animals and plants. Modulating receptor signalling is critical for appropriate cellular responses but the mechanisms ensuring this are poorly understood. Here, we show that signalling by plant receptors for pathogen-associated molecular patterns (PAMPs) in immunity and CLAVATA3/EMBRYO SURROUNDING REGION-RELATED peptides (CLEp) in development uses a similar regulatory module. In the absence of ligand, signalling is dampened through association with specific type-2C protein phosphatases. Upon activation, PAMP and CLEp receptors phosphorylate divergent cytosolic kinases, which, in turn, phosphorylate the phosphatases, thereby promoting receptor signalling. Our work reveals a regulatory circuit shared between immune and developmental receptor signalling, which may have broader important implications for plant receptor kinase-mediated signalling in general.
AB - Ligand recognition by cell-surface receptors underlies development and immunity in both animals and plants. Modulating receptor signalling is critical for appropriate cellular responses but the mechanisms ensuring this are poorly understood. Here, we show that signalling by plant receptors for pathogen-associated molecular patterns (PAMPs) in immunity and CLAVATA3/EMBRYO SURROUNDING REGION-RELATED peptides (CLEp) in development uses a similar regulatory module. In the absence of ligand, signalling is dampened through association with specific type-2C protein phosphatases. Upon activation, PAMP and CLEp receptors phosphorylate divergent cytosolic kinases, which, in turn, phosphorylate the phosphatases, thereby promoting receptor signalling. Our work reveals a regulatory circuit shared between immune and developmental receptor signalling, which may have broader important implications for plant receptor kinase-mediated signalling in general.
UR - http://www.scopus.com/inward/record.url?scp=85128063672&partnerID=8YFLogxK
U2 - 10.1038/s41477-022-01134-w
DO - 10.1038/s41477-022-01134-w
M3 - Article
C2 - 35422079
AN - SCOPUS:85128063672
VL - 8
SP - 356
EP - 365
JO - Nature Plants
JF - Nature Plants
SN - 2055-026X
IS - 4
ER -