A dual functional redox enzyme maturation protein for respiratory and assimilatory nitrate reductases in bacteria

Benjamin Pinchbeck, Manuel Soriano-Laguna, Matthew Sullivan, Victor Luque-Almagro, Gary Rowley, Stuart J Ferguson, M.D. Roldan, David Richardson, Andrew Gates (Lead Author)

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Abstract

Nitrate is available to microbes in many environments due to sustained use of inorganic fertilizers on agricultural soils and many bacterial and archaeal lineages have the capacity to express respiratory (Nar) and assimilatory (Nas) nitrate reductases to utilize this abundant respiratory substrate and nutrient for growth. Here we show that in the denitrifying bacterium Paracoccus denitrificans, NarJ serves as a chaperone for both the anaerobic respiratory nitrate reductase (NarG) and the assimilatory nitrate reductase (NasC), the latter of which is active during both aerobic and anaerobic nitrate assimilation. Bioinformatic analysis suggests that the potential for this previously unrecognized role for NarJ in functional maturation of other cytoplasmic molybdenum-dependent nitrate reductases may be phylogenetically widespread as many bacteria contain both Nar and Nas systems.
Original languageEnglish
Pages (from-to)1592-1603
JournalMolecular Microbiology
Volume111
Issue number6
Early online date15 Mar 2019
DOIs
Publication statusPublished - Jun 2019

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