A human lens model of cortical cataract: Ca2+-induced protein loss, vimentin cleavage and opacification

Julie Sanderson, Julia M. Marcantonio, George Duncan

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95 Citations (Scopus)

Abstract

PURPOSE. Cortical cataract in humans is associated with Ca2+ overload and protein loss, and although animal models of cataract have implicated Ca2+-activated proteases in this process, it remains to be determined whether the human lens responds in this manner to conditions of Ca2+ overload. The purpose of these experiments was to investigate Ca2+-induced opacification and proteolysis in the organ-cultured human lens. METHODS. Donor human lenses were cultured in Eagle's minimum essential medium (EMEM) for up to 14 days. The Ca2+ ionophore ionomycin was used to induce a Ca2+ overload. Lenses were loaded with [H-3]-amino acids for 48 hours. After a 24-hour control efflux period, lenses were cultured in control EMEM (Ca2+ 1.8 mM), EMEM + 5 mu M ionomycin, or EMEM + 5 mu M ionomycin + 5 mM EGTA (Ca2+
Original languageEnglish
Pages (from-to)2255-2261
Number of pages7
JournalInvestigative Ophthalmology & Visual Science
Volume41
Issue number8
Publication statusPublished - 2000

Keywords

  • TRANSGLUTAMINASE
  • CALPAIN
  • SENILE CATARACTS
  • LIGHT-SCATTERING
  • PROTEOLYSIS
  • CALCIUM-INDUCED OPACIFICATION

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