A Lotus japonicus cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation

Jaslyn E M M Wong, Marcin Nadzieja, Lene H Madsen, Christoph A Bücherl, Svend Dam, Niels N Sandal, Daniel Couto, Paul Derbyshire, Mette Uldum-Berentsen, Sina Schroeder, Veit Schwämmle, Fábio C S Nogueira, Mette H Asmussen, Søren Thirup, Simona Radutoiu, Mickaël Blaise, Kasper R Andersen, Frank L H Menke, Cyril Zipfel, Jens Stougaard

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The establishment of nitrogen-fixing root nodules in legume-rhizobia symbiosis requires an intricate communication between the host plant and its symbiont. We are, however, limited in our understanding of the symbiosis signaling process. In particular, how membrane-localized receptors of legumes activate signal transduction following perception of rhizobial signaling molecules has mostly remained elusive. To address this, we performed a coimmunoprecipitation-based proteomics screen to identify proteins associated with Nod factor receptor 5 (NFR5) in Lotus japonicus. Out of 51 NFR5-associated proteins, we focused on a receptor-like cytoplasmic kinase (RLCK), which we named NFR5-interacting cytoplasmic kinase 4 (NiCK4). NiCK4 associates with heterologously expressed NFR5 in Nicotiana benthamiana, and directly binds and phosphorylates the cytoplasmic domains of NFR5 and NFR1 in vitro. At the cellular level, Nick4 is coexpressed with Nfr5 in root hairs and nodule cells, and the NiCK4 protein relocates to the nucleus in an NFR5/NFR1-dependent manner upon Nod factor treatment. Phenotyping of retrotransposon insertion mutants revealed that NiCK4 promotes nodule organogenesis. Together, these results suggest that the identified RLCK, NiCK4, acts as a component of the Nod factor signaling pathway downstream of NFR5.

Original languageEnglish
Pages (from-to)14339-14348
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number28
Early online date25 Jun 2019
Publication statusPublished - 9 Jul 2019

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