TY - JOUR
T1 - A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat
AU - Kolodziej, Markus C.
AU - Singla, Jyoti
AU - Sánchez-Martín, Javier
AU - Zbinden, Helen
AU - Šimková, Hana
AU - Karafiátová, Miroslava
AU - Doležel, Jaroslav
AU - Gronnier, Julien
AU - Poretti, Manuel
AU - Glauser, Gaétan
AU - Zhu, Wangsheng
AU - Köster, Philipp
AU - Zipfel, Cyril
AU - Wicker, Thomas
AU - Krattinger, Simon G
AU - Keller, Beat
PY - 2021/2/11
Y1 - 2021/2/11
N2 - Plasma membrane-associated and intracellular proteins and protein complexes play a pivotal role in pathogen recognition and disease resistance signaling in plants and animals. The two predominant protein families perceiving plant pathogens are receptor-like kinases and nucleotide binding-leucine-rich repeat receptors (NLR), which often confer race-specific resistance. Leaf rust is one of the most prevalent and most devastating wheat diseases. Here, we clone the race-specific leaf rust resistance gene Lr14a from hexaploid wheat. The cloning of Lr14a is aided by the recently published genome assembly of ArinaLrFor, an Lr14a-containing wheat line. Lr14a encodes a membrane-localized protein containing twelve ankyrin (ANK) repeats and structural similarities to Ca2+-permeable non-selective cation channels. Transcriptome analyses reveal an induction of genes associated with calcium ion binding in the presence of Lr14a. Haplotype analyses indicate that Lr14a-containing chromosome segments were introgressed multiple times into the bread wheat gene pool, but we find no variation in the Lr14a coding sequence itself. Our work demonstrates the involvement of an ANK-transmembrane (TM)-like type of gene family in race-specific disease resistance in wheat. This forms the basis to explore ANK-TM-like genes in disease resistance breeding.
AB - Plasma membrane-associated and intracellular proteins and protein complexes play a pivotal role in pathogen recognition and disease resistance signaling in plants and animals. The two predominant protein families perceiving plant pathogens are receptor-like kinases and nucleotide binding-leucine-rich repeat receptors (NLR), which often confer race-specific resistance. Leaf rust is one of the most prevalent and most devastating wheat diseases. Here, we clone the race-specific leaf rust resistance gene Lr14a from hexaploid wheat. The cloning of Lr14a is aided by the recently published genome assembly of ArinaLrFor, an Lr14a-containing wheat line. Lr14a encodes a membrane-localized protein containing twelve ankyrin (ANK) repeats and structural similarities to Ca2+-permeable non-selective cation channels. Transcriptome analyses reveal an induction of genes associated with calcium ion binding in the presence of Lr14a. Haplotype analyses indicate that Lr14a-containing chromosome segments were introgressed multiple times into the bread wheat gene pool, but we find no variation in the Lr14a coding sequence itself. Our work demonstrates the involvement of an ANK-transmembrane (TM)-like type of gene family in race-specific disease resistance in wheat. This forms the basis to explore ANK-TM-like genes in disease resistance breeding.
KW - Ankyrin Repeat/genetics
KW - Basidiomycota/pathogenicity
KW - Disease Resistance/genetics
KW - Gene Expression Regulation, Plant
KW - Gene Pool
KW - Gene Silencing
KW - Genes, Plant/genetics
KW - Haplotypes
KW - Membrane Proteins/genetics
KW - Mutagenesis
KW - Plant Breeding
KW - Plant Diseases/genetics
KW - Plant Proteins/genetics
KW - Tobacco/genetics
KW - Triticum/genetics
UR - http://www.scopus.com/inward/record.url?scp=85101087954&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-20777-x
DO - 10.1038/s41467-020-20777-x
M3 - Article
C2 - 33574268
VL - 12
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 956
ER -