A method of extraction of DNA from birch

DE Howland; RP Oliver; AJ Davy

doi:10.1007/BF02672010

A method of extraction of DNA from birch

DE Howland, RP Oliver, AJ Davy

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A method of DNA extraction is given which is suitable for use with birch (Betula spp.) material collected from natural populations. Such material is frequently old and insect-damaged, and contains high levels of polyphenols. The method relies on repeated extraction of the material with a high molarity urea phosphate buffer, and yields DNA suitable for RFLP analysis.

Original language	English
Pages (from-to)	340-344
Number of pages	5
Journal	Plant Molecular Biology Reporter
Volume	9
Issue number	4
DOIs	https://doi.org/10.1007/BF02672010
Publication status	Published - 1991

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10.1007/BF02672010

Cite this

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title = "A method of extraction of DNA from birch",

abstract = "A method of DNA extraction is given which is suitable for use with birch (Betula spp.) material collected from natural populations. Such material is frequently old and insect-damaged, and contains high levels of polyphenols. The method relies on repeated extraction of the material with a high molarity urea phosphate buffer, and yields DNA suitable for RFLP analysis.",

author = "DE Howland and RP Oliver and AJ Davy",

year = "1991",

doi = "10.1007/BF02672010",

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journal = "Plant Molecular Biology Reporter",

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AU - Oliver, RP

AU - Davy, AJ

PY - 1991

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AB - A method of DNA extraction is given which is suitable for use with birch (Betula spp.) material collected from natural populations. Such material is frequently old and insect-damaged, and contains high levels of polyphenols. The method relies on repeated extraction of the material with a high molarity urea phosphate buffer, and yields DNA suitable for RFLP analysis.

U2 - 10.1007/BF02672010

DO - 10.1007/BF02672010

M3 - Article

SN - 0735-9640

VL - 9

SP - 340

EP - 344

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 4

ER -