TY - JOUR
T1 - A microbe associated with sleep revealed by a novel systems genetic analysis of the microbiome in collaborative cross mice
AU - Bubier, Jason A.
AU - Philip, Vivek M.
AU - Quince, Christopher
AU - Campbell, James
AU - Zhou, Yanjiao
AU - Vishnivetskaya, Tatiana
AU - Duvvuru, Suman
AU - Blair, Rachel Hageman
AU - Ndukum, Juliet
AU - Donohue, Kevin D.
AU - Foster, Carmen M.
AU - Mellert, David J.
AU - Weinstock, George
AU - Culiat, Cymbeline T.
AU - O'Hara, Bruce F.
AU - Palumbo, Anthony V.
AU - Podar, Mircea
AU - Chesler, Elissa J.
N1 - Funding Information: This research was sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U. S. Department of Energy, under contract DE-AC05-00OR22725. J.A.B. was funded by National Institute on Drug Abuse grant U01 DA-043809.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - The microbiome influences health and disease through complex networks of host genetics, genomics, microbes, and environment. Identifying the mechanisms of these interactions has remained challenging. Systems genetics in laboratory mice (Mus musculus) enables data-driven discovery of biological network components and mechanisms of host-microbial interactions underlying disease phenotypes. To examine the interplay among the whole host genome, transcriptome, and microbiome, we mapped QTL and correlated the abundance of cecal messenger RNA, luminal microflora, physiology, and behavior in a highly diverse Collaborative Cross breeding population. One such relationship, regulated by a variant on chromosome 7, was the association of Odoribacter (Bacteroidales) abundance and sleep phenotypes. In a test of this association in the BKS.Cg-Dock7m +/+ Leprdb/J mouse model of obesity and diabetes, known to have abnormal sleep and colonization by Odoribacter, treatment with antibiotics altered sleep in a genotype-dependent fashion. The many other relationships extracted from this study can be used to interrogate other diseases, microbes, and mechanisms.
AB - The microbiome influences health and disease through complex networks of host genetics, genomics, microbes, and environment. Identifying the mechanisms of these interactions has remained challenging. Systems genetics in laboratory mice (Mus musculus) enables data-driven discovery of biological network components and mechanisms of host-microbial interactions underlying disease phenotypes. To examine the interplay among the whole host genome, transcriptome, and microbiome, we mapped QTL and correlated the abundance of cecal messenger RNA, luminal microflora, physiology, and behavior in a highly diverse Collaborative Cross breeding population. One such relationship, regulated by a variant on chromosome 7, was the association of Odoribacter (Bacteroidales) abundance and sleep phenotypes. In a test of this association in the BKS.Cg-Dock7m +/+ Leprdb/J mouse model of obesity and diabetes, known to have abnormal sleep and colonization by Odoribacter, treatment with antibiotics altered sleep in a genotype-dependent fashion. The many other relationships extracted from this study can be used to interrogate other diseases, microbes, and mechanisms.
KW - Behavior
KW - Bioinformatics
KW - Genetics
KW - Genomics
KW - Sleep
UR - http://www.scopus.com/inward/record.url?scp=85081082543&partnerID=8YFLogxK
U2 - 10.1534/genetics.119.303013
DO - 10.1534/genetics.119.303013
M3 - Article
C2 - 31896565
AN - SCOPUS:85081082543
VL - 214
SP - 719
EP - 733
JO - Genetics
JF - Genetics
SN - 1943-2631
IS - 3
ER -