A mixture of Lactobacillus species isolated from traditional fermented foods promote recovery from antibiotic-induced intestinal disruption in mice

Ying Shi, Xi Zhao-Wilson, Zhao Jianxin, Zhang Hao, Qixiao Zhai, Arjan Narbad, Wei Chen

Research output: Contribution to journalArticle

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Abstract

Aims: This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice.

Methods and Results: Administration of the antibiotic ampicillin for 2 weeks decreased microbial community diversity, induced caecum tumefaction and increased gut permeability in mice. Application of a probiotic cocktail of four Lactobacillus species (JUP-Y4) modulated the microbiota community structure and promoted the abundance of potentially beneficial bacteria such as Akkermansia. Ampicillin administration led to a decline in Bacteroidetes from 46.6±3.91% to 0.264±0.0362%; the addition of JUP-Y4 restored this to 41.4±2.87%. This probiotic supplementation was more effective than natural restoration, where the levels of Bacteroidetes were only restored to 29.3%±2.07%. Interestingly, JUP-Y4 treatment was more effective in the restoration of microbiota in faecal samples than in caecal samples. JUP-Y4 also significantly reduced the levels of D-lactate and endotoxin (lipopolysaccharide, LPS) in the serum of mice, and increased the expression of tight-junction proteins while reducing the production of inflammatory cytokines (TNF-α, IL-6, MCP-1, IFN-γ and IL-1β) in the ileum and the colon of antibiotic-treated mice.

Conclusions: JUP-Y4 not only promoted recovery from antibiotic-induced gut dysbiosis, but also enhanced the function of the gut barrier, reduced inflammation, and lowered levels of circulating endotoxin in mice.

Significance and Impact of Study: Consumption of a mixture of Lactobacillus species may encourage faster recovery from antibiotic-induced gut dysbiosis and gut microbiota-related immune disturbance.
Original languageEnglish
Pages (from-to)842–854
JournalJournal of Applied Microbiology
Volume124
Issue number3
Early online date3 Jan 2018
DOIs
Publication statusPublished - Mar 2018

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