A modified form of low-density lipoprotein with increased electronegative charge is present in rheumatoid arthritis synovial fluid

Letian Dai, Zhi Zhang, Paul G. Winyard, Karl Gaffney, Hugh Jones, David R. Blake, Christopher J. Morris

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are pro-inflammatory factors in the pathogenesis of rheumatoid arthritis. During inflammation, the amount of low-density lipoprotein (LDL) in the inflamed joint is increased. LDL is known to be susceptible to oxidation by ROS. Oxidized LDL may serve as a mediator for joint damage, further exacerbating the inflammatory process. LDL isolated from synovial fluid and plasma from individual patients (paired samples) with rheumatoid arthritis or osteoarthritis was characterized by crossed immunoelectrophoresis. On analysis by this technique, synovial fluid LDL from most patients with rheumatoid arthritis contained two peaks: one corresponding to normal plasma native LDL, and the other having an increased electrophoretic mobility associated with oxidized LDL. Paired plasma LDL samples contained native LDL alone, as did paired synovial fluid and plasma LDL from patients with osteoarthritis. Thus, in addition to native LDL, a second form of LDL was shown to be present in rheumatoid synovial fluid, which had properties consistent with those of oxidized LDL.

Original languageEnglish
Pages (from-to)705-710
Number of pages6
JournalFree Radical Biology & Medicine
Volume22
Issue number4
DOIs
Publication statusPublished - 1997

Keywords

  • Aged
  • Arthritis, Rheumatoid/blood
  • Electrochemistry
  • Female
  • Humans
  • Immunoelectrophoresis, Two-Dimensional
  • Lipoproteins, LDL/blood
  • Male
  • Middle Aged
  • Osteoarthritis/blood
  • Oxidation-Reduction
  • Reactive Oxygen Species/metabolism
  • Synovial Fluid/metabolism

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