A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.
|Number of pages||4|
|Journal||Nucleosides, Nucleotides & Nucleic Acids|
|Publication status||Published - 2001|
|Event||XIVth International Roundtable on Nucleosides, Nucleotides and their Biological Applications - San Francisco, California, United States|
Duration: 10 Sep 2000 → 14 Sep 2000