A new facile method for spin-labeling of oligonucleotides

Thorsten Strube; Olav Schiemann; Fraser Macmillan; Thomas Prisner; Joachim W. Engels

doi:10.1081/NCN-100002534

A new facile method for spin-labeling of oligonucleotides

Thorsten Strube, Olav Schiemann, Fraser Macmillan, Thomas Prisner, Joachim W. Engels

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

Original language	English
Pages (from-to)	1271-1274
Number of pages	4
Journal	Nucleosides, Nucleotides & Nucleic Acids
Volume	20
Issue number	4-7
DOIs	https://doi.org/10.1081/NCN-100002534
Publication status	Published - 2001
Event	XIVth International Roundtable on Nucleosides, Nucleotides and their Biological Applications - San Francisco, United States Duration: 10 Sep 2000 → 14 Sep 2000

Access to Document

10.1081/NCN-100002534

Cite this

@article{c285725ebd4c4ab2bf68c0f512e1a5d9,

title = "A new facile method for spin-labeling of oligonucleotides",

abstract = "A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.",

author = "Thorsten Strube and Olav Schiemann and Fraser Macmillan and Thomas Prisner and Engels, {Joachim W.}",

year = "2001",

doi = "10.1081/NCN-100002534",

language = "English",

volume = "20",

pages = "1271--1274",

journal = "Nucleosides, Nucleotides & Nucleic Acids",

issn = "1525-7770",

publisher = "Marcel Dekker Inc.",

number = "4-7",

note = "XIVth International Roundtable on Nucleosides, Nucleotides and their Biological Applications ; Conference date: 10-09-2000 Through 14-09-2000",

}

TY - JOUR

T1 - A new facile method for spin-labeling of oligonucleotides

AU - Strube, Thorsten

AU - Schiemann, Olav

AU - Macmillan, Fraser

AU - Prisner, Thomas

AU - Engels, Joachim W.

PY - 2001

Y1 - 2001

N2 - A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

AB - A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.

U2 - 10.1081/NCN-100002534

DO - 10.1081/NCN-100002534

M3 - Article

VL - 20

SP - 1271

EP - 1274

JO - Nucleosides, Nucleotides & Nucleic Acids

JF - Nucleosides, Nucleotides & Nucleic Acids

SN - 1525-7770

IS - 4-7

T2 - XIVth International Roundtable on Nucleosides, Nucleotides and their Biological Applications

Y2 - 10 September 2000 through 14 September 2000

ER -