A noncanonical vacuolar sugar transferase required for biosynthesis of antimicrobial defense compounds in oat

Anastasia Orme, Thomas Louveau, Michael J. Stephenson, Ingo Appelhagen, Rachel Melton, Jitender Cheema, Yan Li, Qiang Zhao, Lei Zhang, Danlin Fan, Qilin Tian, Robert J. Vickerstaff, Tim Langdon, Bin Han, Anne Osbourn

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Plants produce an array of natural products with important ecological functions. These compounds are often decorated with oligosaccharide groups that influence bioactivity, but the biosynthesis of such sugar chains is not well understood. Triterpene glycosides (saponins) are a large family of plant natural products that determine important agronomic traits, as exemplified by avenacins, antimicrobial defense compounds produced by oats. Avenacins have a branched trisaccharide moiety consisting of L-arabinose linked to 2 D-glucose molecules that is critical for antifungal activity. Plant natural product glycosylation is usually performed by uridine diphosphate-dependent glycosyltransferases (UGTs). We previously characterized the arabinosyltransferase that initiates the avenacin sugar chain; however, the enzymes that add the 2 remaining D-glucose molecules have remained elusive. Here we characterize the enzymes that catalyze these last 2 glucosylation steps. AsUGT91G16 is a classical cytosolic UGT that adds a 1,2-linked D-glucose molecule to L-arabinose. Unexpectedly, the enzyme that adds the final 1,4-linked D-glucose (AsTG1) is not a UGT, but rather a sugar transferase belonging to Glycosyl Hydrolase family 1 (GH1). Unlike classical UGTs, AsTG1 is vacuolar. Analysis of oat mutants reveals that AsTG1 corresponds to Sad3, a previously uncharacterized locus shown by mutation to be required for avenacin biosynthesis. AsTG1 and AsUGT91G16 form part of the avenacin biosynthetic gene cluster. Our demonstration that a vacuolar transglucosidase family member plays a critical role in triterpene biosynthesis highlights the importance of considering other classes of carbohydrate-active enzymes in addition to UGTs as candidates when elucidating pathways for the biosynthesis of glycosylated natural products in plants.
Original languageEnglish
Pages (from-to)27105-27114
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number52
Early online date5 Dec 2019
DOIs
Publication statusPublished - 26 Dec 2019

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