A novel fluorescent probe for NAD-consuming enzymes

Giulia Pergolizzi; Julea N. Butt; Richard P. Bowater; Gerd K. Wagner

doi:10.1039/c1cc15499k

A novel fluorescent probe for NAD-consuming enzymes

Giulia Pergolizzi
, Julea N. Butt
, Richard P. Bowater
, Gerd K. Wagner

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

11 Downloads (Pure)

Abstract

A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.

Original language	English
Pages (from-to)	12655-12657
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	47
DOIs	https://doi.org/10.1039/c1cc15499k
Publication status	Published - 2011

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10.1039/c1cc15499k

Cite this

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title = "A novel fluorescent probe for NAD-consuming enzymes",

abstract = "A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.",

author = "Giulia Pergolizzi and Butt, \{Julea N.\} and Bowater, \{Richard P.\} and Wagner, \{Gerd K.\}",

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journal = "Chemical Communications",

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AU - Pergolizzi, Giulia

AU - Butt, Julea N.

AU - Bowater, Richard P.

AU - Wagner, Gerd K.

PY - 2011

Y1 - 2011

N2 - A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.

AB - A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.

U2 - 10.1039/c1cc15499k

DO - 10.1039/c1cc15499k

M3 - Article

SN - 1359-7345

VL - 47

SP - 12655

EP - 12657

JO - Chemical Communications

JF - Chemical Communications

IS - 47

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