TY - JOUR
T1 - A radiation hybrid transcript map of the mouse genome
AU - Avner, Philip
AU - Bruls, Thomas
AU - Poras, Isabelle
AU - Eley, Lorraine
AU - Gas, Shahinaz
AU - Ruiz, Patricia
AU - Wiles, Michael V.
AU - Sousa-Nunes, Rita
AU - Kettleborough, Ross
AU - Rana, Amer
AU - Morissette, Jean
AU - Bentley, Liz
AU - Goldsworthy, Michelle
AU - Haynes, Alison
AU - Herbert, Eifion
AU - Southam, Lorraine
AU - Lehrach, Hans
AU - Weissenbach, Jean
AU - Manenti, Giacomo
AU - Rodriguez-Tome, Patricia
AU - Beddington, Rosa
AU - Dunwoodie, Sally
AU - Cox, Roger D.
N1 - Funding Information:
We dedicate this article to our friend and colleague Rosa Beddington (March 23, 1956–May 18, 2001), a scientist of great biological insight. This work was supported by EEC Contract PL 962414. We thank B. Gorick and the Human Genome Mapping Project at Hinxton, UK, for help with the replication of the 7.5-dpc mouse endoderm library; A.M. Mallon and S. Greenaway of the informatics group at the Medical Research Council, Harwell; and V. Taghavi and E. Sartory for technical assistance in marker typing at MRC Harwell.
PY - 2001
Y1 - 2001
N2 - Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done. Species for which radiation hybrid-based transcript maps have been established include human, rat, mouse, dog, cat and zebrafish. We have established a comprehensive first-generation-placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence- tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.
AB - Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done. Species for which radiation hybrid-based transcript maps have been established include human, rat, mouse, dog, cat and zebrafish. We have established a comprehensive first-generation-placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence- tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.
UR - http://www.scopus.com/inward/record.url?scp=0034785444&partnerID=8YFLogxK
U2 - 10.1038/ng1001-194
DO - 10.1038/ng1001-194
M3 - Article
C2 - 11586301
AN - SCOPUS:0034785444
VL - 29
SP - 194
EP - 200
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 2
ER -