A radiation hybrid transcript map of the mouse genome

Philip Avner, Thomas Bruls, Isabelle Poras, Lorraine Eley, Shahinaz Gas, Patricia Ruiz, Michael V. Wiles, Rita Sousa-Nunes, Ross Kettleborough, Amer Rana, Jean Morissette, Liz Bentley, Michelle Goldsworthy, Alison Haynes, Eifion Herbert, Lorraine Southam, Hans Lehrach, Jean Weissenbach, Giacomo Manenti, Patricia Rodriguez-TomeRosa Beddington, Sally Dunwoodie, Roger D. Cox

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done. Species for which radiation hybrid-based transcript maps have been established include human, rat, mouse, dog, cat and zebrafish. We have established a comprehensive first-generation-placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence- tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.

Original languageEnglish
Pages (from-to)194-200
Number of pages7
JournalNature Genetics
Issue number2
Publication statusPublished - 2001

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