A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

M. Jawed Iqbal; Alastair Forbes; Shahin H. Valyani; Timothy P. Corbishley; Mark L. Wilkinson

doi:10.1016/0039-128X(88)90055-4

A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

M. Jawed Iqbal, Alastair Forbes, Shahin H. Valyani, Timothy P. Corbishley, Mark L. Wilkinson

Research output: Contribution to journal › Article › peer-review

Abstract

A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.

Original language	English
Pages (from-to)	599-607
Number of pages	9
Journal	Steroids
Volume	51
Issue number	5-6
DOIs	https://doi.org/10.1016/0039-128X(88)90055-4
Publication status	Published - 1988

Keywords

Animals
Enzyme-Linked Immunosorbent Assay
Ligands
Rabbits
Reproducibility of Results
Sex Hormone-Binding Globulin

Access to Document

10.1016/0039-128X(88)90055-4

Cite this

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title = "A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin",

abstract = "A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.",

keywords = "Animals, Enzyme-Linked Immunosorbent Assay, Ligands, Rabbits, Reproducibility of Results, Sex Hormone-Binding Globulin",

author = "Iqbal, {M. Jawed} and Alastair Forbes and Valyani, {Shahin H.} and Corbishley, {Timothy P.} and Wilkinson, {Mark L.}",

year = "1988",

doi = "10.1016/0039-128X(88)90055-4",

language = "English",

volume = "51",

pages = "599--607",

journal = "Steroids",

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T1 - A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

AU - Iqbal, M. Jawed

AU - Forbes, Alastair

AU - Valyani, Shahin H.

AU - Corbishley, Timothy P.

AU - Wilkinson, Mark L.

PY - 1988

Y1 - 1988

N2 - A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.

AB - A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.

KW - Animals

KW - Enzyme-Linked Immunosorbent Assay

KW - Ligands

KW - Rabbits

KW - Reproducibility of Results

KW - Sex Hormone-Binding Globulin

U2 - 10.1016/0039-128X(88)90055-4

DO - 10.1016/0039-128X(88)90055-4

M3 - Article

C2 - 3242179

VL - 51

SP - 599

EP - 607

JO - Steroids

JF - Steroids

SN - 0039-128X

IS - 5-6

ER -