A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

M. Jawed Iqbal, Alastair Forbes, Shahin H. Valyani, Timothy P. Corbishley, Mark L. Wilkinson

Research output: Contribution to journalArticle

Abstract

A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.
Original languageEnglish
Pages (from-to)599-607
Number of pages9
JournalSteroids
Volume51
Issue number5-6
DOIs
Publication statusPublished - 1988

Keywords

  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Ligands
  • Rabbits
  • Reproducibility of Results
  • Sex Hormone-Binding Globulin

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