A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration

Aram Saeed; Nora Francini; Lisa White; James Dixon; Toby Gould; Hassan Rashidi; Racha Cheikh Al Ghanami; Veronika Hruschka; Heinz Redl; Brian R. Saunders; Cameron Alexander; Kevin M. Shakesheff

doi:10.1002/adma.201403626

A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration

Aram Saeed, Nora Francini, Lisa White, James Dixon, Toby Gould, Hassan Rashidi, Racha Cheikh Al Ghanami, Veronika Hruschka, Heinz Redl, Brian R. Saunders, Cameron Alexander, Kevin M. Shakesheff

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Abstract

A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.

Original language	English
Pages (from-to)	662–668
Number of pages	7
Journal	Advanced Materials
Volume	27
Issue number	4
Early online date	29 Nov 2014
DOIs	https://doi.org/10.1002/adma.201403626
Publication status	Published - 27 Jan 2015

Keywords

3D scaffolds
biomaterials
magnetic particles
stem cells
thermoresponsive polymers

Access to Document

10.1002/adma.201403626

Published manuscript
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Cite this

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title = "A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration",

abstract = "A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.",

keywords = "3D scaffolds, biomaterials, magnetic particles, stem cells, thermoresponsive polymers",

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T1 - A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration

AU - Saeed, Aram

AU - Francini, Nora

AU - White, Lisa

AU - Dixon, James

AU - Gould, Toby

AU - Rashidi, Hassan

AU - Al Ghanami, Racha Cheikh

AU - Hruschka, Veronika

AU - Redl, Heinz

AU - Saunders, Brian R.

AU - Alexander, Cameron

AU - Shakesheff, Kevin M.

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Y1 - 2015/1/27

N2 - A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.

AB - A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.

KW - 3D scaffolds

KW - biomaterials

KW - magnetic particles

KW - stem cells

KW - thermoresponsive polymers

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