Accurate determination of microbial diversity from 454 pyrosequencing data

Christopher Quince, Anders Lanzén, Thomas P. Curtis, Russell J. Davenport, Neil Hall, Ian M. Head, L. Fiona Read, William T. Sloan

Research output: Contribution to journalArticlepeer-review

827 Citations (Scopus)


We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

Original languageEnglish
Pages (from-to)639-641
Number of pages3
JournalNature Methods
Issue number9
Publication statusPublished - 2009

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