AePUb promoter length modulates gene expression in Aedes aegypti

Michelle A.E. Anderson, Philip T. Leftwich, Ray Wilson, Leonela Z. Carabajal Paladino, Sanjay Basu, Sara Rooney, Zach N. Adelman, Luke Alphey

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.

Original languageEnglish
Article number20352
JournalScientific Reports
Volume13
DOIs
Publication statusPublished - 21 Nov 2023
Externally publishedYes

Cite this