AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

Delphine Sicard; Samy Cecioni; Maksym Iazykov; Yann Chevolot; Susan E. Matthews; Jean-Pierre Praly; Eliane Souteyrand; Anne Imberty; Sébastien Vidal; Magali Phaner-Goutorbe

doi:10.1039/C1CC13097H

AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

Delphine Sicard, Samy Cecioni, Maksym Iazykov, Yann Chevolot, Susan E. Matthews, Jean-Pierre Praly, Eliane Souteyrand, Anne Imberty, Sébastien Vidal, Magali Phaner-Goutorbe

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59 Citations (Scopus)

Abstract

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

Original language	English
Pages (from-to)	9483-9485
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	33
DOIs	https://doi.org/10.1039/C1CC13097H
Publication status	Published - 2011

Access to Document

10.1039/C1CC13097H

Cite this

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title = "AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters",

abstract = "Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.",

author = "Delphine Sicard and Samy Cecioni and Maksym Iazykov and Yann Chevolot and Matthews, {Susan E.} and Jean-Pierre Praly and Eliane Souteyrand and Anne Imberty and S{\'e}bastien Vidal and Magali Phaner-Goutorbe",

year = "2011",

doi = "10.1039/C1CC13097H",

language = "English",

volume = "47",

pages = "9483--9485",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "33",

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TY - JOUR

T1 - AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

AU - Sicard, Delphine

AU - Cecioni, Samy

AU - Iazykov, Maksym

AU - Chevolot, Yann

AU - Matthews, Susan E.

AU - Praly, Jean-Pierre

AU - Souteyrand, Eliane

AU - Imberty, Anne

AU - Vidal, Sébastien

AU - Phaner-Goutorbe, Magali

PY - 2011

Y1 - 2011

N2 - Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

AB - Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

U2 - 10.1039/C1CC13097H

DO - 10.1039/C1CC13097H

M3 - Article

VL - 47

SP - 9483

EP - 9485

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 33

ER -