AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

Delphine Sicard, Samy Cecioni, Maksym Iazykov, Yann Chevolot, Susan E. Matthews, Jean-Pierre Praly, Eliane Souteyrand, Anne Imberty, Sébastien Vidal, Magali Phaner-Goutorbe

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.
Original languageEnglish
Pages (from-to)9483-9485
Number of pages3
JournalChemical Communications
Volume47
Issue number33
DOIs
Publication statusPublished - 2011

Cite this