AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

Delphine Sicard; Samy Cecioni; Maksym Iazykov; Yann Chevolot; Susan E. Matthews; Jean-Pierre Praly; Eliane Souteyrand; Anne Imberty; Sébastien Vidal; Magali Phaner-Goutorbe

doi:10.1039/C1CC13097H

AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

Delphine Sicard, Samy Cecioni, Maksym Iazykov, Yann Chevolot, Susan E. Matthews, Jean-Pierre Praly, Eliane Souteyrand, Anne Imberty, Sébastien Vidal, Magali Phaner-Goutorbe

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

Original language	English
Pages (from-to)	9483-9485
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	33
DOIs	https://doi.org/10.1039/C1CC13097H
Publication status	Published - 2011

Access to Document

10.1039/C1CC13097H

Cite this

Sicard, Delphine ; Cecioni, Samy ; Iazykov, Maksym ; Chevolot, Yann ; Matthews, Susan E. ; Praly, Jean-Pierre ; Souteyrand, Eliane ; Imberty, Anne ; Vidal, Sébastien ; Phaner-Goutorbe, Magali. / AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters. In: Chemical Communications. 2011 ; Vol. 47, No. 33. pp. 9483-9485.

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title = "AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters",

abstract = "Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.",

author = "Delphine Sicard and Samy Cecioni and Maksym Iazykov and Yann Chevolot and Matthews, {Susan E.} and Jean-Pierre Praly and Eliane Souteyrand and Anne Imberty and S{\'e}bastien Vidal and Magali Phaner-Goutorbe",

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doi = "10.1039/C1CC13097H",

language = "English",

volume = "47",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters. / Sicard, Delphine; Cecioni, Samy; Iazykov, Maksym; Chevolot, Yann; Matthews, Susan E.; Praly, Jean-Pierre; Souteyrand, Eliane; Imberty, Anne; Vidal, Sébastien; Phaner-Goutorbe, Magali.

In: Chemical Communications, Vol. 47, No. 33, 2011, p. 9483-9485.

Research output: Contribution to journal › Article › peer-review

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T1 - AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

AU - Sicard, Delphine

AU - Cecioni, Samy

AU - Iazykov, Maksym

AU - Chevolot, Yann

AU - Matthews, Susan E.

AU - Praly, Jean-Pierre

AU - Souteyrand, Eliane

AU - Imberty, Anne

AU - Vidal, Sébastien

AU - Phaner-Goutorbe, Magali

PY - 2011

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N2 - Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

AB - Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

U2 - 10.1039/C1CC13097H

DO - 10.1039/C1CC13097H

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