Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

Pierre Buscaill, Nattapong Sanguankiattichai, Yoon Joo Lee, Jiorgos Kourelis, Gail Preston, Renier A. L. van der Hoorn

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The lengthy process to generate transformed plants is a limitation in current research on the interactions of the model plant pathogen Pseudomonas syringae with plant hosts. Here we present an easy method called agromonas, where we quantify P. syringae growth in agroinfiltrated leaves of Nicotiana benthamiana using a cocktail of antibiotics to select P. syringae on plates. As a proof of concept, we demonstrate that transient expression of PAMP receptors reduces bacterial growth, and that transient depletion of a host immune gene and transient expression of a type-III effector increase P. syringae growth in agromonas assays. We show that we can rapidly achieve structure−function analysis of immune components and test the function of immune hydrolases. The agromonas method is easy, fast and robust for routine disease assays with various Pseudomonas strains without transforming plants or bacteria. The agromonas assay offers a reliable approach for further comprehensive analysis of plant immunity.

Original languageEnglish
Pages (from-to)831-840
Number of pages10
JournalPlant Journal
Volume105
Issue number3
Early online date12 Jan 2021
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Agrobacterium
  • disease assay
  • Nicotiana benthamiana
  • plant immunity
  • Pseudomonas syringae
  • technical advance

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