Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype

Miren Iturriza Gómara, David Cubitt, Ulrich Desselberger, Jim Gray

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Abstract

Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp→Asn) correlated with the change in ability to serotype these G2 strains.
Original languageEnglish
Pages (from-to)3796-3798
Number of pages3
JournalJournal of Clinical Microbiology
Volume39
Issue number10
DOIs
Publication statusPublished - 2001

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