Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype

Miren Iturriza Gómara; David Cubitt; Ulrich Desselberger; Jim Gray

doi:10.1128/JCM.39.10.3796-3798.2001

Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype

Miren Iturriza Gómara
, David Cubitt
, Ulrich Desselberger
, Jim Gray

Norwich Medical School

Research output: Contribution to journal › Article

147 Citations (Scopus)

Abstract

Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp→Asn) correlated with the change in ability to serotype these G2 strains.

Original language	English
Pages (from-to)	3796-3798
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	10
DOIs	https://doi.org/10.1128/JCM.39.10.3796-3798.2001
Publication status	Published - 2001

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10.1128/JCM.39.10.3796-3798.2001

Cite this

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title = "Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype",

abstract = "Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp→Asn) correlated with the change in ability to serotype these G2 strains.",

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AU - Gómara, Miren Iturriza

AU - Cubitt, David

AU - Desselberger, Ulrich

AU - Gray, Jim

PY - 2001

Y1 - 2001

N2 - Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp→Asn) correlated with the change in ability to serotype these G2 strains.

AB - Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp→Asn) correlated with the change in ability to serotype these G2 strains.

U2 - 10.1128/JCM.39.10.3796-3798.2001

DO - 10.1128/JCM.39.10.3796-3798.2001

M3 - Article

SN - 0095-1137

VL - 39

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

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