Amplification and overexpression of E2F3 in human bladder cancer

Andrew Feber, Jeremy Clark, Graham Goodwin, Andrew R Dodson, Paul H Smith, Anne Fletcher, Sandra Edwards, Penny Flohr, Alison Falconer, Toby Roe, Gyula Kovacs, Nening Dennis, Cyril Fisher, Richard Wooster, Robert Huddart, Christopher S Foster, Colin S Cooper

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134 Citations (Scopus)

Abstract

We demonstrate that, in human bladder cancer, amplification of the E2F3 gene, located at 6p22, is associated with overexpression of its encoded mRNA transcripts and high levels of expression of E2F3 protein. Immunohistochemical analyses of E2F3 protein levels have established that around one-third (33/101) of primary transitional cell carcinomas of the bladder overexpress nuclear E2F3 protein, with the proportion of tumours containing overexpressed nuclear E2F3 increasing with tumour stage and grade. When considered together with the established role of E2F3 in cell cycle progression, these results suggest that the E2F3 gene represents a candidate bladder cancer oncogene that is activated by DNA amplification and overexpression.
Original languageEnglish
Pages (from-to)1627-30
Number of pages4
JournalOncogene
Volume23
Issue number8
DOIs
Publication statusPublished - 26 Feb 2004

Keywords

  • Base Sequence
  • Carcinoma, Transitional Cell
  • Cell Line, Tumor
  • Cell Nucleus
  • Chromosome Mapping
  • Chromosomes, Human, Pair 6
  • DNA, Neoplasm
  • E2F3 Transcription Factor
  • Gene Amplification
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Neoplasm Staging
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Transcription Factors
  • Urinary Bladder Neoplasms

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