@article{9a53fcbc22fe4fbe89783795994a6126,
title = "An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes",
abstract = "We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.",
keywords = "Salmonella, Thermolysates, Whole-genome sequencing, iNTS",
author = "Perez-Sepulveda, {Blanca M.} and Darren Heavens and Pulford, {Caisey V.} and Predeus, {Alexander V.} and Ross Low and Hermione Webster and Dykes, {Gregory F.} and Christian Schudoma and Will Rowe and James Lipscombe and Chris Watkins and Benjamin Kumwenda and Neil Shearer and Karl Costigan and Baker, {Kate S.} and Feasey, {Nicholas A.} and Hinton, {Jay C. D.} and Neil Hall and {The 10KSG consortium}",
note = "Funding Information: The project was supported by both a Global Challenges Research Fund (GCRF) data & resources grant BBS/OS/GC/000009D and the BBSRC Core Capability Grant to the Earlham Institute BB/CCG1720/1 and Core Strategic Programme Grant BBS/E/T/000PR9817. This project was partly supported by the Wellcome Trust Senior Investigator Award (106914/Z/15/Z) to Jay C. D. Hinton.",
year = "2021",
month = dec,
day = "21",
doi = "10.1186/s13059-021-02536-3",
language = "English",
volume = "22",
journal = "Genome Biology",
issn = "1474-760X",
publisher = "BioMed Central",
number = "1",
}