Tendinopathy is a prevalent, highly debilitating condition, with poorly defined aetiology. A wide range of clinical treatments have been proposed, with systematic reviews largely supporting shock wave therapy or eccentric exercise. Characterising these treatments has demonstrated both generate perturbations within tendon at a frequency of approximately 812Hz. Consequently, it is hypothesised that loading in this frequency range initiates increased anabolic tenocyte behaviour, promoting tendon repair. The primary aim of this study is to investigate the effects of 10Hz perturbations on tenocyte metabolism, comparing gene expression in response to a 10Hz and 1Hz loading profile. Tenocytes from healthy and tendinopathic human tendons were seeded into 3D collagen gels and subjected to 15 mins cyclic strain at 10Hz or 1Hz. Tenocytes from healthy tendon showed increased expression of all analysed genes in response to loading, with significantly increased expression of inflammatory and degradative genes with 10Hz, relative to 1Hz loading. By contrast, whilst the response of tenocytes from tendinopathy tendon also increased with 10Hz loading, the overall response profile was more varied and less intense, possibly indicative of an altered healing response. Through inhibition of the pathway, IL1 was shown to be involved in the degradative and catabolic response of cells to high frequency loading, abrogating the loading response. This study has demonstrated for the first time that loading at a frequency of 10Hz may enhance the metabolic response of tenocytes by initiating an immediate degradatory and inflammatory cell response through the IL1 pathway, perhaps as an initial stage of tendon healing.
|Journal||Scandinavian Journal of Medicine and Science in Sports|
|Early online date||18 May 2019|
|Publication status||Published - Oct 2019|
- inflammatory response
- high frequency
- 3D collagen
- cyclic strain