TY - JOUR
T1 - An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
AU - Pandey, Pooja
AU - Leary, Alexandre Y.
AU - Tumtas, Yasin
AU - Savage, Zachary
AU - Dagvadorj, Bayantes
AU - Duggan, Cian
AU - Yuen, Enoch L. H.
AU - Sanguankiattichai, Nattapong
AU - Tan, Emily
AU - Khandare, Virendrasinh
AU - Connerton, Amber J.
AU - Yunusov, Temur
AU - Madalinski, Mathias
AU - Mirkin, Federico Gabriel
AU - Schornack, Sebastian
AU - Dagdas, Yasin
AU - Kamoun, Sophien
AU - Bozkurt, Tolga O.
PY - 2021/8/23
Y1 - 2021/8/23
N2 - Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.
AB - Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.
U2 - 10.7554/eLife.65285
DO - 10.7554/eLife.65285
M3 - Article
VL - 10
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e65285
ER -