Analysis of Aging in Caenorhabditis elegans

Deepti S. Wilkinson, Rebecca C. Taylor, Andrew Dillin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter is dedicated to the study of aging in Caenorhabditis elegans (C. elegans). The assays are divided into two sections. In the first section, we describe detailed protocols for performing life span analysis in solid and liquid medium. In the second section, we describe various assays for measuring age-related changes. Our laboratory has been involved in several fruitful collaborations with non- C. elegans researchers keen on testing a role for their favorite gene in modulating aging (Carrano et al., 2009; Dong et al., 2007; Raices et al., 2008; Wolff et al., 2006). But even with the guidance of trained worm biologists, this undertaking can be daunting. We hope that this chapter will serve as a worthy compendium for those researchers who may or may not have immediate access to laboratories studying C. elegans.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Chapter12
Pages353-381
Number of pages29
Volume107
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume107
ISSN (Print)0091-679X

Keywords

  • Abnormality
  • Aging
  • Knockdown
  • Lifespan
  • Muscle decline
  • Survival curve

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