Analysis of multiple haloarchaeal genomes suggests that the quinone-dependent respiratory nitric oxide reductase is an important source of nitrous oxide in hypersaline environments

Javier Torregrosa-Crespo, Pedro González-Torres, Vanesa Bautista, Julia M. Esclapez, Carmen Pire, Monica Camacho, María José Bonete, David J. Richardson, Nicholas J. Watmough, Rosa María Martínez-Espinosa

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Abstract

Microorganisms, including Bacteria and Archaea, play a key role in denitrification, which is the major mechanism by which fixed nitrogen returns to the atmosphere from soil and water. Whilst the enzymology of denitrification is well understood in Bacteria, the details of the last two reactions in this pathway, which catalyse the reduction of nitric oxide (NO) via nitrous oxide (N2O) to nitrogen (N2), are little studied in Archaea, and hardly at all in haloarchaea. This
work describes an extensive interspecies analysis of both complete and draft haloarchaeal genomes aimed at identifying the genes that encode respiratory nitric oxide reductases (Nors). The study revealed that the only nor gene found in haloarchaea is one that encodes a single subunit quinone dependent Nor homologous to the qNor found in bacteria. This surprising discovery is considered in terms of our emerging understanding of haloarchaeal bioenergetics and NO management.
Original languageEnglish
Pages (from-to)788–796
Number of pages9
JournalEnvironmental Microbiology Reports
Volume9
Issue number6
Early online date19 Sep 2017
DOIs
Publication statusPublished - Dec 2017

Keywords

  • Bioinformatics
  • Denitrification
  • Haloarchaea
  • Hypersaline environments
  • Nitric Oxide
  • Nitrous Oxide
  • Nitric Oxide Reductase

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