Analysis of the bacterial communities associated with two ant-plant symbioses

Ryan F. Seipke, Jörg Barke, Darren Heavens, Douglas W. Yu, Matthew I. Hutchings

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
7 Downloads (Pure)

Abstract

Insect fungiculture is practiced by ants, termites, beetles, and gall midges and it has been suggested to be widespread among plant–ants. Some of the insects engaged in fungiculture, including attine ants and bark beetles, are known to use symbiotic antibiotic-producing actinobacteria to protect themselves and their fungal cultivars against infection. In this study, we analyze the bacterial communities on the cuticles of the plant–ant genera Allomerus and Tetraponera using deep sequencing of 16S rRNA. Allomerus ants cultivate fungus as a building material to strengthen traps for prey, while Tetraponera ants cultivate fungus as a food source. We report that Allomerus and Tetraponera microbiomes contain >75% Proteobacteria and remarkably the bacterial phyla that dominate their cuticular microbiomes are very similar despite their geographic separation (South America and Africa, respectively). Notably, antibiotic-producing actinomycete bacteria represent a tiny fraction of the cuticular microbiomes of both Allomerus and Tetraponera spp. and instead they are dominated by γ-proteobacteria Erwinia and Serratia spp. Both these phyla are known to contain antibiotic-producing species which might therefore play a protective role in these ant–plant systems.
Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalMicrobiologyOpen
Volume2
Issue number2
Early online date17 Feb 2013
DOIs
Publication statusPublished - 1 Apr 2013

Keywords

  • 16S pyrosequencing
  • Allomerus
  • fungus-growing ants
  • microbiome
  • Tetraponera

Cite this