Anti-inflammatory activity of extracts and a saponin isolated from Melilotus elegans

K Asres, S Gibbons, E Hana, F Bucar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The crude methanol extract of Melilotus elegans Ser. (Fabaceae), a plant widely used in Ethiopian traditional medicine for the treatment of asthma, haemorrhoid and lacerated wounds showed a significant anti-inflammatory activity against carrageenin-induced rat paw oedema. At a dose corresponding to 333.3 mg per kg body weight of dry plant material, the methanol extract displayed a strong inhibitory effect that was comparable to the inhibitory effect of 1 mg/kg of indomethacin in the same test system. Bioassay guided fractionation of the alcoholic extract led to the isolation of an oleanene-type triterpene saponin identified as azukisaponin V (1) ((3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranosyl(1 --> 2)-beta-D-glucuronopyranosyl]-soyasapogenol B). The structure of the compound was identified by using MS and extensive one- and two-dimensional NMR experiments (1H, 13C, COSY, HMQC, HMBC and NOESY). One hour after injection of carrageenin, inhibition of oedema exerted by 1 was approximately ten times higher than that of indomethacin on a molar basis.

Original languageEnglish
Pages (from-to)310-312
Number of pages3
JournalDie Pharmazie
Volume60
Issue number4
Publication statusPublished - Apr 2005

Keywords

  • Animals
  • Anti-Inflammatory Agents/isolation & purification
  • Carbohydrate Sequence
  • Carrageenan
  • Chromatography, High Pressure Liquid
  • Edema/chemically induced
  • Ethiopia
  • Hydrolysis
  • Medicine, African Traditional
  • Melilotus/chemistry
  • Molecular Sequence Data
  • Plant Extracts/pharmacology
  • Plant Leaves/chemistry
  • Rats
  • Saponins/isolation & purification

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