Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea

Natalya Doroshenko, Stephen Rimmer, Richard Hoskins, Prashant Garg, Thomas Swift, Hannah L. M. Spencer, Rianne M. Lord, Maria Katsikogianni, David Pownall, Sheila MacNeil, C. W. Ian Douglas, Joanna Shepherd

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Abstract

Microbial keratitis can arise from penetrating injuries to the cornea. Corneal trauma promotes bacterial attachment and biofilm growth, which decrease the effectiveness of antimicrobials against microbial keratitis. Improved therapeutic efficacy can be achieved by reducing microbial burden prior to antimicrobial therapy. This paper assesses a highly-branched poly(N-isopropyl acrylamide) with vancomycin end groups (HB-PNIPAM-van), for reducing bacterial attachment and biofilm formation. The polymer lacked antimicrobial activity against Staphylococcus aureus, but significantly inhibited biofilm formation (p = 0.0008) on plastic. Furthermore, pre-incubation of S. aureus cells with HB-PNIPAM-van reduced cell attachment by 50% and application of HB-PNIPAM-van to infected ex vivo rabbit corneas caused a 1-log reduction in bacterial recovery, compared to controls (p = 0.002). In conclusion, HB-PNIPAM-van may be a useful adjunct to antimicrobial therapy in the treatment of corneal infections.
Original languageEnglish
Pages (from-to)2101-2109
Number of pages9
JournalBiomaterials Science
Volume6
DOIs
Publication statusPublished - 4 Jun 2018

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