TY - JOUR
T1 - Antimicrobial resistance and co-selection phenomenon in Listeria spp. recovered from food and food production environments
AU - Kovacevic, Jovana
AU - Sagert, Jason
AU - Wozniak, Anna
AU - Gilmour, Matthew W.
AU - Allen, Kevin J.
N1 - Funding Information:
This work was supported by the National Sciences and Engineering Research Council of Canada . We would like to acknowledge British Columbia Centre for Disease Control for donating bacterial isolates, and the Canadian National Microbiology Laboratory for performing serotyping. We also extend our thanks to Dr. Lili Mesak and Wenqian Yuan for their technical assistance.
PY - 2013/6
Y1 - 2013/6
N2 - Antimicrobial resistance (AMR), co-selection phenomenon, and the relationship between reduced susceptibility (RSC) to ciprofloxacin (CIP) and resistance to other antimicrobials in Listeria spp. (n = 103) recovered from food processing environments (FPE) and food were investigated. Resistance of Listeria monocytogenes and other listeriae, respectively, to cefoxitin (FOX; 98% vs. 88%), CIP (7% vs. 4%), clindamycin (CLI; 33% vs. 59%) and tetracycline (6% vs. 8%) was observed, as was RSC to CIP (67% vs. 57%) and CLI (65% vs. 41%). L. monocytogenes also possessed RSC to linezolid (LZD; 6%), rifampicin (2%) and streptomycin (6%), with other listeriae displaying RSC to chloramphenicol (4%). L. monocytogenes serotype 1/2a (90%) isolates were more frequently resistant or possessed RSC to CIP compared to serotype 4b (55%) (p = 0.015). When eight strains were experimentally adapted to high concentrations of CIP, co-selection occurred as MICs to benzalkonium chloride (BAC) increased (n = 5), gentamicin MICs remained the same (n = 6) or increased 2-fold (n = 2), and led to RSC to LZD (n = 1) and resistance to CLI (n = 8). Overall, levels of resistance/RSC to CIP in food chain isolates, particularly 1/2a, are concerning. Further, reduced sensitivity to disparate antimicrobials following CIP exposure highlights the need for increased knowledge of co-selection phenomenon linked with antimicrobial agents.
AB - Antimicrobial resistance (AMR), co-selection phenomenon, and the relationship between reduced susceptibility (RSC) to ciprofloxacin (CIP) and resistance to other antimicrobials in Listeria spp. (n = 103) recovered from food processing environments (FPE) and food were investigated. Resistance of Listeria monocytogenes and other listeriae, respectively, to cefoxitin (FOX; 98% vs. 88%), CIP (7% vs. 4%), clindamycin (CLI; 33% vs. 59%) and tetracycline (6% vs. 8%) was observed, as was RSC to CIP (67% vs. 57%) and CLI (65% vs. 41%). L. monocytogenes also possessed RSC to linezolid (LZD; 6%), rifampicin (2%) and streptomycin (6%), with other listeriae displaying RSC to chloramphenicol (4%). L. monocytogenes serotype 1/2a (90%) isolates were more frequently resistant or possessed RSC to CIP compared to serotype 4b (55%) (p = 0.015). When eight strains were experimentally adapted to high concentrations of CIP, co-selection occurred as MICs to benzalkonium chloride (BAC) increased (n = 5), gentamicin MICs remained the same (n = 6) or increased 2-fold (n = 2), and led to RSC to LZD (n = 1) and resistance to CLI (n = 8). Overall, levels of resistance/RSC to CIP in food chain isolates, particularly 1/2a, are concerning. Further, reduced sensitivity to disparate antimicrobials following CIP exposure highlights the need for increased knowledge of co-selection phenomenon linked with antimicrobial agents.
KW - Antimicrobial resistance
KW - Co-selection
KW - Cross-resistance
KW - Efflux pump
KW - Listeria monocytogenes
KW - Listeria spp.
UR - http://www.scopus.com/inward/record.url?scp=84875662766&partnerID=8YFLogxK
U2 - 10.1016/j.fm.2013.01.002
DO - 10.1016/j.fm.2013.01.002
M3 - Article
C2 - 23541198
AN - SCOPUS:84875662766
VL - 34
SP - 319
EP - 327
JO - Food Microbiology
JF - Food Microbiology
SN - 0740-0020
IS - 2
ER -
