Antimycobacterials from Lovage root (Ligusticum officinale Koch)

Juan David Guzman, Dimitrios Evangelopoulos, Antima Gupta, Jose M. Prieto, Simon Gibbons, Sanjib Bhakta

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The n-hexane extract of Lovage root was found to significantly inhibit the growth of both Mycobacterium smegmatis mc2155 and Mycobacterium bovis BCG, and therefore a bioassay-guided isolation strategy was undertaken. (Z)-Ligustilide, (Z)-3-butylidenephthalide, (E)-3-butylidenephthalide, 3-butylphthalide, α-prethapsenol, falcarindiol, levistolide A, psoralen and bergapten were isolated by chromatographic techniques, characterized by NMR spectroscopy and MS, and evaluated for their growth inhibition activity against Mycobacterium tuberculosis H37Rv using the whole-cell phenotypic spot culture growth inhibition assay (SPOTi). Cytotoxicity against RAW 264.7 murine macrophage cells was employed for assessing their degree of selectivity. Falcarindiol was the most potent compound with a minimum inhibitory concentration (MIC) value of 20 mg/L against the virulent H37Rv strain; however, it was found to be cytotoxic with a half-growth inhibitory concentration (GIC50) in the same order of magnitude (SI < 1). Interestingly the sesquiterpene alcohol α-prethapsenol was found to inhibit the growth of the pathogenic mycobacteria with an MIC value of 60 mg/L, being more specific towards mycobacteria than mammalian cells (SI ~ 2). Colony forming unit analysis at different concentrations of this phytochemical showed mycobacteriostatic mode of action.

Original languageEnglish
Pages (from-to)993-998
Number of pages6
JournalPhytotherapy Research
Volume27
Issue number7
Early online date16 Aug 2012
DOIs
Publication statusPublished - Jul 2013

Keywords

  • α-prethapsenol
  • cytotoxicity
  • Ligusticum officinale Koch
  • Lovage
  • tuberculosis

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