Apertures in the Clostridium sporogenes spore coat and exosporium align to facilitate emergence of the vegetative cell

Jason Brunt, Kathryn L. Cross, Michael W. Peck

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Clostridium sporogenes forms highly heat resistant endospores, enabling this bacterium to survive adverse conditions. Subsequently, spores may germinate, giving rise to vegetative cells that multiply and lead to food spoilage. Electron microscopy was used to visualise changes in spore structures during germination, emergence and outgrowth. C. sporogenes spores were surrounded by an exosporium that was oval in shape and typically 3 μm in length. An aperture of 0.3–0.4 μm was observed at one end of the exosporium. The rupture of the spore coats occurs adjacent to the opening in the exosporium. The germinated cell emerges through this hole in the spore coat and then through the pre-existing aperture in the exosporium, before eventually being released, leaving behind a largely intact exosporium with an enlarged aperture (0.7–1.0 μm) and coat shell. The formation of this aperture, its function and its alignment with the spore coat is discussed.
Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalFood Microbiology
Volume51
DOIs
Publication statusPublished - Oct 2015

Cite this