Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling

Erik A. van der Biezen; Jongho Sun; Mark J. Coleman; Mervyn J. Bibb; Jonathan D. G. Jones

doi:10.1073/pnas.97.7.3747

Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling

Erik A. van der Biezen, Jongho Sun, Mark J. Coleman, Mervyn J. Bibb, Jonathan D. G. Jones

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Abstract

Arabidopsis RPP5 is a member of a large class of pathogen resistance genes encoding nucleotide-binding sites and leucine-rich repeat domains. Yeast two-hybrid analysis showed that RPP5 specifically interacts with At-RSH1, an Arabidopsis RelA/SpoT homolog. In Escherichia coli, RelA and SpoT determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotides of the bacterial stringent response. Functional analysis in E. coli and in Streptomyces coelicolor A3 (2) showed that At-RSH1 confers phenotypes associated with (p)ppGpp synthesis. We characterized two additional Arabidopsis RelA/SpoT homologs, At-RSH2 and At-RSH3. At-RSH genes may regulate a rapid plant (p)ppGpp-mediated response to pathogens and other stresses.

Original language	English
Pages (from-to)	3747-3752
Number of pages	6
Journal	Proceedings of the National Academy of Sciences
Volume	97
Issue number	7
DOIs	https://doi.org/10.1073/pnas.97.7.3747
Publication status	Published - 28 Mar 2000

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10.1073/pnas.97.7.3747

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title = "Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling",

abstract = "Arabidopsis RPP5 is a member of a large class of pathogen resistance genes encoding nucleotide-binding sites and leucine-rich repeat domains. Yeast two-hybrid analysis showed that RPP5 specifically interacts with At-RSH1, an Arabidopsis RelA/SpoT homolog. In Escherichia coli, RelA and SpoT determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotides of the bacterial stringent response. Functional analysis in E. coli and in Streptomyces coelicolor A3 (2) showed that At-RSH1 confers phenotypes associated with (p)ppGpp synthesis. We characterized two additional Arabidopsis RelA/SpoT homologs, At-RSH2 and At-RSH3. At-RSH genes may regulate a rapid plant (p)ppGpp-mediated response to pathogens and other stresses.",

author = "{van der Biezen}, {Erik A.} and Jongho Sun and Coleman, {Mark J.} and Bibb, {Mervyn J.} and Jones, {Jonathan D. G.}",

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TY - JOUR

T1 - Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling

AU - van der Biezen, Erik A.

AU - Sun, Jongho

AU - Coleman, Mark J.

AU - Bibb, Mervyn J.

AU - Jones, Jonathan D. G.

PY - 2000/3/28

Y1 - 2000/3/28

N2 - Arabidopsis RPP5 is a member of a large class of pathogen resistance genes encoding nucleotide-binding sites and leucine-rich repeat domains. Yeast two-hybrid analysis showed that RPP5 specifically interacts with At-RSH1, an Arabidopsis RelA/SpoT homolog. In Escherichia coli, RelA and SpoT determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotides of the bacterial stringent response. Functional analysis in E. coli and in Streptomyces coelicolor A3 (2) showed that At-RSH1 confers phenotypes associated with (p)ppGpp synthesis. We characterized two additional Arabidopsis RelA/SpoT homologs, At-RSH2 and At-RSH3. At-RSH genes may regulate a rapid plant (p)ppGpp-mediated response to pathogens and other stresses.

AB - Arabidopsis RPP5 is a member of a large class of pathogen resistance genes encoding nucleotide-binding sites and leucine-rich repeat domains. Yeast two-hybrid analysis showed that RPP5 specifically interacts with At-RSH1, an Arabidopsis RelA/SpoT homolog. In Escherichia coli, RelA and SpoT determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotides of the bacterial stringent response. Functional analysis in E. coli and in Streptomyces coelicolor A3 (2) showed that At-RSH1 confers phenotypes associated with (p)ppGpp synthesis. We characterized two additional Arabidopsis RelA/SpoT homologs, At-RSH2 and At-RSH3. At-RSH genes may regulate a rapid plant (p)ppGpp-mediated response to pathogens and other stresses.

U2 - 10.1073/pnas.97.7.3747

DO - 10.1073/pnas.97.7.3747

M3 - Article

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EP - 3752

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -