TY - JOUR
T1 - Are Sinc and the PrP gene congruent? Evidence from PrP gene analysis in Sinc congenic mice
AU - Hunter, Nora
AU - Dann, Janine C.
AU - Bennett, Alan D.
AU - Somerville, Robert A.
AU - McConnell, Irene
AU - Hope, James
PY - 1992
Y1 - 1992
N2 - Congenic mouse strains VM/Dk and VM-Sinc(s7)/Dk differ at the Sinc gene, which controls the incubation period of scrapie in mice; VM/Dk mice are Sinc(p7p7) and VM-Sin(s7)/Dk mice are Sinc(s7s7). Restriction fragment length polymorphism and DNA sequencing analysis demonstrated that the PrP genes also differ in these strains, confirming the close genetic linkage of Sinc and PrP. Using the restriction enzyme HhaI, we have shown that at least 100 kb of DNA flanking the PrP gene differs between the two strains, and therefore the congruence of the Sinc and PrP genes is still not certain.
AB - Congenic mouse strains VM/Dk and VM-Sinc(s7)/Dk differ at the Sinc gene, which controls the incubation period of scrapie in mice; VM/Dk mice are Sinc(p7p7) and VM-Sin(s7)/Dk mice are Sinc(s7s7). Restriction fragment length polymorphism and DNA sequencing analysis demonstrated that the PrP genes also differ in these strains, confirming the close genetic linkage of Sinc and PrP. Using the restriction enzyme HhaI, we have shown that at least 100 kb of DNA flanking the PrP gene differs between the two strains, and therefore the congruence of the Sinc and PrP genes is still not certain.
UR - http://www.scopus.com/inward/record.url?scp=0026739272&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-73-10-2751
DO - 10.1099/0022-1317-73-10-2751
M3 - Article
C2 - 1357080
AN - SCOPUS:0026739272
SN - 0022-1317
VL - 73
SP - 2751
EP - 2755
JO - Journal of General Virology
JF - Journal of General Virology
IS - 10
ER -