Automatic diagnosis of tuberculosis disease based on Plasmonic ELISA and color-based image classification

Kamal AbuHassan, Noremylia Bakhori, Norzila Kusnin, Umi Azmi, Marzia Tania, Benjamin Evans, Nor Yusof, Alamgir Hossain

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)
14 Downloads (Pure)


Tuberculosis (TB) remains one of the most devastating infectious diseases and its treatment efficiency is majorly influenced by the stage at which infection with the TB bacterium is diagnosed. The available methods for TB diagnosis are either time consuming, costly or not efficient. This study employs a signal generation mechanism for biosensing, known as Plasmonic ELISA, and computational intelligence to facilitate automatic diagnosis of TB. Plasmonic ELISA enables the detection of a few molecules of analyte by the incorporation of smart nanomaterials for better sensitivity of the developed detection system. The computational system uses k-means clustering and thresholding for image segmentation. This paper presents the results of the classification performance of the Plasmonic ELISA imaging data by using various types of classifiers. The five-fold cross-validation results show high accuracy rate (>97%) in classifying TB images using the entire data set. Future work will focus on developing an intelligent mobile-enabled expert system to diagnose TB in real-time. The intelligent system will be clinically validated and tested in collaboration with healthcare providers in Malaysia.
Original languageEnglish
Title of host publicationEngineering in Medicine and Biology Society (EMBC), 2017 39th Annual International Conference of the IEEE
PublisherThe Institute of Electrical and Electronics Engineers (IEEE)
ISBN (Electronic)978-1-5090-2809-2
ISBN (Print)978-1-5090-2810-8
Publication statusPublished - 14 Sep 2017


  • Image color analysis
  • Image segmentation
  • Plasmons
  • Feature extraction
  • Testing
  • Image classification
  • Vegetation

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