Bacterial resistance modifying tetrasaccharide agents from Ipomoea murucoides

Lilia Chérigo, Rogelio Pereda-Miranda, Simon Gibbons

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Abstract

As part of an ongoing project to identify oligosaccharides which modulate bacterial multidrug resistance, the CHCl3-soluble extract from flowers of a Mexican arborescent morning glory, Ipomoea murucoides, through preparative-scale recycling HPLC, yielded five lipophilic tetrasaccharide inhibitors of Staphylococcus aureus multidrug efflux pumps, murucoidins XII–XVI (1–5). The macrocyclic lactone-type structures for these linear hetero-tetraglycoside derivatives of jalapinolic acid were established by spectroscopic methods. These compounds were tested for in vitro antibacterial and resistance modifying activity against strains of Staphylococcus aureus possessing multidrug resistance efflux mechanisms. Only murucoidin XIV (3) displayed antimicrobial activity against SA-1199B (MIC 32 μg/ml), a norfloxacin-resistant strain that over-expresses the NorA MDR efflux pump. The four microbiologically inactive (MIC > 512 μg/ml) tetrasaccharides increased norfloxacin susceptibility of this strain by 4-fold (8 μg/ml from 32 μg/ml) at concentrations of 25 μg/ml, while murucoidin XIV (3) exerted the same potentiation effect at a concentration of 5 μg/ml.
Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalPhytochemistry
Volume70
Issue number2
DOIs
Publication statusPublished - Jan 2009

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