BamA β16C strand and periplasmic turns are critical for outer membrane protein insertion and assembly

Yinghong Gu, Yi Zeng, Zhongshan Wang, Changjiang Dong

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9 Citations (Scopus)
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Abstract

Outer membrane β-barrel proteins play important roles in importing nutrients, exporting wastes and conducting signals in Gram-negative bacteria, mitochondria and chloroplasts. The outer membrane proteins are inserted and assembled into the outer membrane by OMP85 family proteins. In Escherichia coli , the b-barrel assembly machinery (BAM) contains four lipoproteins BamB, BamC, BamD and BamE, and one outer membrane protein BamA, forming a "top hat"-like structure. Structural and functional studies of the E. coli BAM machinery have revealed that the rotation of periplasmic ring may trigger the barrel b1C-b6C scissor-like movement that promote the unfolded outer membrane protein insertion without using ATP. Here we report the BamA C-terminal barrel structure of Salmonella enterica Typhimurium str. LT2 and functional assays, which reveal that the BamA's C-terminal residue Trp, the b16C strand of the barrel and the periplasmic turns are critical for the functionality of BamA. These findings indicate that the unique b16C and the periplasmic turns of BamA are important for the out membrane insertion and assembly. The periplasmic turns might mediate the rotation of the periplasmic ring to the scissor-like movement of BamA b1C-b6C, triggering the outer membrane protein insertion. These results are important for understanding the outer membrane protein insertion in Gram-negative bacteria, as well as in mitochondria and chloroplasts.
Original languageEnglish
Pages (from-to)3951-3961
Number of pages11
JournalBiochemical Journal
Volume474
Issue number23
Early online date3 Oct 2017
DOIs
Publication statusPublished - 21 Nov 2017

Keywords

  • Outer membrane biogenesis
  • Gram negative bacteria
  • membrane protein structure
  • outer membrane protein insertion and assembly

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