@article{5de011f2b2674e78bf9e7927d7fb1b91,
title = "Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae",
abstract = "Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes to host proteins; few characterized NLRs have been shown to recognize multiple effectors. The barley (Hordeum vulgare) NLR gene Mildew locus a (Mla) has undergone functional diversification, and the proteins encoded by different Mla alleles recognize host-adapted isolates of barley powdery mildew (Blumeria graminis f. sp. hordei [Bgh]). Here, we show that Mla3 also confers resistance to the rice blast fungus Magnaporthe oryzae in a dosage-dependent manner. Using a forward genetic screen, we discovered that the recognized effector from M. oryzae is Pathogenicity toward Weeping Lovegrass 2 (Pwl2), a host range determinant factor that prevents M. oryzae from infecting weeping lovegrass (Eragrostis curvula). Mla3 has therefore convergently evolved the capacity to recognize effectors from diverse pathogens.",
author = "Brabham, {Helen J.} and {De La Cruz}, {Diana G{\'o}mez} and Vincent Were and Motoki Shimizu and Hiromasa Saitoh and Inmaculada Hern{\'a}ndez-Pinz{\'o}n and Phon Green and Jennifer Lorang and Koki Fujisaki and Kazuhiro Sato and Istv{\'a}n Moln{\'a}r and Hana {\v S}imkov{\'a} and Jaroslav Dole{\v z}el and James Russell and Jodie Taylor and Matthew Smoker and Gupta, {Yogesh Kumar} and Tom Wolpert and Talbot, {Nicholas J.} and Ryohei Terauchi and Moscou, {Matthew J.}",
note = "Funding Information: Funding for this research includes United Kingdom Research and Innovation-Biotechnology and Biological Sciences Research Council Norwich Research Park Doctoral Training Partnership (grant no. BB/M011216/1 to JR) and Institute Strategic Programme (grant no. BB/P012574/1 to M.J.M. and BBS/E/J/000 PR9795 to M.J.M.), European Regional Development Fund (grant no. CZ.02.1.01/0.0/0.0/16_019/0000827 to I.M., J.D., and H.{\v S}.), Perry Foundation (H.J.B.), Japan Society for the Promotion of Science 2018 Summer Programme (H.J.B.), Gatsby Charitable Foundation (M.J.M. and N.J.T.), and United States Department of Agriculture-Agricultural Research Service CRIS #5062-21220-025-000D (M.J.M.).",
year = "2024",
month = feb,
doi = "10.1093/plcell/koad266",
language = "English",
volume = "36",
pages = "447--470",
journal = "The Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "2",
}