Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate

Deborah Püngel, Agatha Treveil, Matthew J Dalby, Shabhonam Caim, Ian J Colquhoun, Catherine Booth, Jennifer Ketskemety, Tamas Korcsmaros, Douwe Van Sinderen, Melissa A E Lawson, Lindsay J Hall

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Abstract

Background: Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe–microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system. Methods: Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR). Results: Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel. Conclusions: These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health.
Original languageEnglish
Article number948
Number of pages17
JournalNutrients
Volume12
Issue number4
DOIs
Publication statusPublished - 29 Mar 2020

Keywords

  • 16S rRNA profiling
  • Bifidobacterium
  • Cross-feeding
  • Diet
  • Early life
  • Exopolysaccharides
  • Metabolomics
  • Model colon

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